Confino E, Friberg J, Silverman S, Dudkiewicz A B, Goldin M, Gleicher N
Obstet Gynecol. 1987 Jul;70(1):134-6.
It has been reported that bacteria may attach to motile spermatozoa, be carried through the cervix and uterus to the fallopian tubes, and cause acute salpingitis. In an attempt to mimic these conditions in vitro, we incubated Escherichia coli, Streptococcus faecalis, Pseudomonas aeruginosa, and Staphylococcus aureus with motile spermatozoa which then were allowed to migrate through a capillary filled with bovine cervical mucus. After satisfactory sperm migration through the mucus, the capillaries were broken and cultured at different distances from the original insemination site. Mucus fractions in proximity to bacterial inocula grew varying amounts of the pathogens. More distal fractions of mucus columns were generally culture-negative even though they contained motile sperm which had been exposed to bacteria. Migration of spermatozoa exposed to bacteria through bovine cervical mucus did not result in enhanced bacterial penetration.
据报道，细菌可能附着于活动精子，通过子宫颈和子宫进入输卵管，并引起急性输卵管炎。为了在体外模拟这些情况，我们将大肠杆菌、粪链球菌、铜绿假单胞菌和金黄色葡萄球菌与活动精子一起孵育，然后让精子通过充满牛宫颈黏液的毛细管迁移。在精子顺利通过黏液后，打破毛细管，并在距原始授精部位不同距离处进行培养。靠近细菌接种物的黏液部分培养出不同数量的病原体。黏液柱更远处的部分通常培养为阴性，尽管它们含有接触过细菌的活动精子。接触过细菌的精子通过牛宫颈黏液的迁移并未导致细菌穿透增强。
