Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA.
Photochem Photobiol. 2019 Sep;95(5):1097-1115. doi: 10.1111/php.13126. Epub 2019 Jul 10.
This review summarizes research on many of the potential applications of photosensitized crosslinking of tissue proteins in surgery and current knowledge of the photochemical mechanisms underlying formation of the covalent protein-protein crosslinks involved. Initially developed to close wounds or reattach tissues, protein photocrosslinking has also been demonstrated to stiffen and strengthen tissues, decrease inflammatory responses and facilitate tissue bioengineering. These treatments appear to result largely from crosslinks within and between collagen molecules in tissue that typically form by an oxygen-dependent mechanism. Surgical applications discussed include sealing wounds in skin, cornea and bowel; reattaching severed nerves, blood vessels and tendons; strengthening cornea and vein; reducing capsular contracture after breast implants; and regenerating joint cartilage.
这篇综述总结了组织蛋白光致敏交联在手术中的许多潜在应用的研究，以及目前对形成涉及的共价蛋白-蛋白交联的光化学机制的了解。最初开发用于闭合伤口或重新连接组织，蛋白质光交联也已被证明可以使组织变硬和增强，减少炎症反应，并促进组织工程。这些治疗方法似乎主要是由于组织内和组织间胶原分子内的交联，这些交联通常通过氧依赖机制形成。讨论的手术应用包括密封皮肤、角膜和肠道的伤口；重新连接切断的神经、血管和肌腱；增强角膜和静脉；减少乳房植入物后的包膜挛缩；以及再生关节软骨。
