Langbein L
Acta Histochem. 1987;81(2):239-42. doi: 10.1016/S0065-1281(87)80018-1.
A simple and inexpensive equipment especially for the dehydration of cells grown on coverslips in a continuous linear gradient is described. On this way it is possible to handle many objects simultaneously. The coverslips, even irregularly formed, are fixed vertically in simple silicon-rubber-holders. The device including a described special holder can also be used for thorough rinsing of EM-grids during the immunocytochemical labelling.
本文描述了一种简单且价格低廉的设备，特别适用于在连续线性梯度中对盖玻片上生长的细胞进行脱水处理。通过这种方式，可以同时处理多个样本。盖玻片，即使形状不规则，也能垂直固定在简单的硅橡胶支架中。该设备包括所述的特殊支架，还可用于在免疫细胞化学标记过程中对电子显微镜网格进行彻底冲洗。
