Takano J, Kinoshita T, Yamane K
Biochem Biophys Res Commun. 1987 Jul 15;146(1):73-9. doi: 10.1016/0006-291x(87)90692-9.
Upstream of the promoter of the Bacillus subtilis alpha-amylase gene (amyE) derived from an alpha-amylase hyper-producing strain, there is an inverted repeat sequence (palindromic sequence), which has a free energy of 21.2 kcal/mol due to the formation of stable stem-loop structure. The role of the palindromic sequence for the expression of amyE was studied using a plasmid encoding the amyE'-'bla (E. coli beta-lactamase) fused gene in an alpha-amylase-deficient B. subtilis mutant as the host. By the presence of the palindromic sequence, the transcription activity of the amyE promoter was enhanced approximately 6 fold by starch (3%) in the medium and was less repressed by glucose.
来源于一株α-淀粉酶高产菌株的枯草芽孢杆菌α-淀粉酶基因（amyE）启动子上游，存在一个反向重复序列（回文序列），由于形成稳定的茎环结构，其自由能为21.2千卡/摩尔。以α-淀粉酶缺陷型枯草芽孢杆菌突变体为宿主，利用编码amyE'-'bla（大肠杆菌β-内酰胺酶）融合基因的质粒，研究了回文序列对amyE表达的作用。由于回文序列的存在，培养基中淀粉（3%）可使amyE启动子的转录活性提高约6倍，且受葡萄糖的抑制作用较小。
