School of Science and Health, University of Western Sydney, Hawkesbury, New South Wales, Australia.
Anat Rec (Hoboken). 2019 Nov;302(11):1985-2002. doi: 10.1002/ar.24176. Epub 2019 Jun 10.
Cells expressing the surface markers CD3, CD4, CD79b, IgM, MHC class II, and ModoUG (nonclassical MHC class I) were detected in red-tailed phascogale tissues using immunohistochemistry, and the appearance and localization of cells observed here was consistent with previous observations in other marsupial species. CD3 cells were first detected at one day postpartum (dpp) in the thymus, followed by ModoUG cells at 5-7 dpp in the thymus and lymph nodes. CD79b cells were first detected at 12-14 dpp in bone marrow, spleen, and lymph nodes. IgM cells were first detected at 12-14 dpp in thymus, bone marrow, spleen, and lymph nodes. MHC class II cells were first detected at 12-14 dpp in thymus, bone marrow, and lymph nodes. CD4 cells were detected in adult thymus and spleen only. The presence of the mature immune cell populations and their localization to characteristic T and B cell zones in mature lymphoid tissues with normal histological structure indicates that red-tailed phascogales develop immunocompetence by the end of pouch life. Anat Rec, 302:1985-2002, 2019. © 2019 American Association for Anatomy.
使用免疫组织化学技术在红尾袋狸组织中检测到表达 CD3、CD4、CD79b、IgM、MHC Ⅱ类和 ModoUG（非经典 MHC Ⅰ类）表面标志物的细胞，这里观察到的细胞的外观和定位与其他有袋动物物种的先前观察结果一致。CD3 细胞首先在产后第 1 天（dpp）在胸腺中检测到，随后在胸腺和淋巴结中在 5-7 dpp 检测到 ModoUG 细胞。CD79b 细胞首先在骨髓、脾脏和淋巴结中在 12-14 dpp 检测到。IgM 细胞首先在胸腺、骨髓、脾脏和淋巴结中在 12-14 dpp 检测到。MHC Ⅱ类细胞首先在胸腺、骨髓和淋巴结中在 12-14 dpp 检测到。CD4 细胞仅在成年胸腺和脾脏中检测到。成熟免疫细胞群的存在及其在具有正常组织学结构的成熟淋巴组织中向特征性 T 和 B 细胞区的定位表明，红尾袋狸在育儿袋生命结束时发育出免疫能力。解剖记录，302：1985-2002，2019. © 2019 年美国解剖学会。
