Yamada T, Sasaki M
Jpn J Cancer Res. 1987 May;78(5):499-504.
An attempt was made, by treatment with 12-O-tetradecanoyl-phorbol-13-acetate (TPA), to induce differentiation in Ph1-positive (PB-1049) and Ph1-negative (LN-1049) B lymphoblastoid cell lines established from a patient with chronic myelogenous leukemia, and a Ph1-positive line (PB-1049-T) derived from a tumor nodule formed by inoculation of PB-1049 cells into nude mice. The changes induced by TPA in all lines were consistent with differentiation towards plasma cells, and included enhanced clustering of floating cells, reduced DNA synthesis, increase of the cytoplasm/nucleus ratio and appearance of a large amount of rough endoplasmic reticulum in the cytoplasm. Immunoglobulin concentration was estimated by ELISA in the supernatant of the three cell lines. Remarkable increases were observed in PB-1049 and PB-1049-T cultures treated with TPA. These results suggest that the Ph1-positive as well as Ph1-negative B lymphoblastoid cell lines examined have the potential to perform the proper functions of B lymphocytes in vitro.
采用12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）处理，试图诱导从一名慢性粒细胞白血病患者建立的Ph1阳性（PB - 1049）和Ph1阴性（LN - 1049）B淋巴母细胞系，以及通过将PB - 1049细胞接种到裸鼠中形成的肿瘤结节衍生的Ph1阳性细胞系（PB - 1049 - T）进行分化。TPA在所有细胞系中诱导的变化与向浆细胞的分化一致，包括悬浮细胞聚集增强、DNA合成减少、细胞质/细胞核比率增加以及细胞质中出现大量粗面内质网。通过ELISA估计三种细胞系上清液中的免疫球蛋白浓度。在用TPA处理的PB - 1049和PB - 1049 - T培养物中观察到显著增加。这些结果表明，所检测的Ph1阳性和Ph1阴性B淋巴母细胞系在体外具有执行B淋巴细胞正常功能的潜力。
