Suppr超能文献

进行CRISPR

"Doing CRISPR": .

作者信息

Dankel Dorothy Jane

机构信息

Universitetet i Bergen, Det Matematisk-naturvitenskapelige Fakultet,

出版信息

Politics Life Sci. 2018 Dec 4;37(2):220-235. doi: 10.1017/pls.2018.14.

Abstract

Salmon farming is a key industry in Norway, with firsthand value of more than 60 billion Norwegian crowns in 2017. The salmon industry is a driving force for biotechnological applications in the marine sector. The recent release of the Atlantic salmon reference genome offers new opportunities to solve major aquaculture bottlenecks that currently limit expansion of the industry. One major bottleneck is the genetic impact of escaped farmed salmon on wild populations. To solve this problem, the industry can use sterile salmon in production. As shown byWargelius et al., sterile salmon can be made by preventing the formation of germ cells through genome editing using the CRISPR-Cas9 method. This approach solves problems of genetic introgression and precocious maturation. However, genome editing of animals, especially for human consumption, raises ethical as well as safety and legal questions. These social and ethical aspects can have tremendous impact in analyzing the final result of salmon farming (e.g., consumer acceptability of a fresh or frozen filet or similar salmon product) but also can be examined "upstream" by describing and assessing the research communities that promote and carry out the science that underpins the salmon industry. Who produces the scientific "facts" that govern the Norwegian aquaculture industry? How do these scientific communities work together? What are the societal impacts of this science? This article uses ethnographical observation and interviews to describe the state-of-the-art of CRISPR gene-editing procedures currently employed in the science and industry collaboration in Norway.

摘要

三文鱼养殖是挪威的一项关键产业,2017年其直接产值超过600亿挪威克朗。三文鱼产业是海洋领域生物技术应用的驱动力。近期大西洋三文鱼参考基因组的发布为解决当前限制该产业扩张的主要水产养殖瓶颈带来了新机遇。一个主要瓶颈是养殖三文鱼逃逸对野生种群的基因影响。为解决这一问题,该产业可在生产中使用不育三文鱼。正如瓦尔格利乌斯等人所示,通过使用CRISPR-Cas9方法进行基因组编辑来阻止生殖细胞的形成,就可以培育出不育三文鱼。这种方法解决了基因渗入和早熟的问题。然而,对动物进行基因组编辑,尤其是用于人类消费,会引发伦理以及安全和法律问题。这些社会和伦理方面不仅会对分析三文鱼养殖的最终结果(例如,消费者对新鲜或冷冻鱼片或类似三文鱼产品的接受度)产生巨大影响,还可以通过描述和评估推动并开展支撑三文鱼产业的科学研究的科研群体,在“上游”层面进行审视。是谁产生了主导挪威水产养殖业的科学“事实”?这些科研群体如何合作?这项科学研究对社会有哪些影响?本文运用人种志观察和访谈来描述挪威目前在科学与产业合作中采用的CRISPR基因编辑程序的最新情况。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验