Haynesworth S E, Carrino D A, Caplan A I
J Biol Chem. 1987 Aug 5;262(22):10574-81.
The core protein of high buoyant density proteoglycans synthesized by chondrocytes in stage 24 chick limb bud mesenchymal cell cultures was cleaved with cyanogen bromide to produce 17 resolvable peptides on sodium dodecyl sulfate-polyacrylamide slab gels. Of these peptides, 10 appear to originate from the chondroitin sulfate-rich region, 2 appear to be derived from the keratan sulfate-rich region, and 5 seem to be derived from the hyaluronic acid-binding region. The peptides from the chondroitin sulfate-rich region are almost all large (200 to 64 kDa). In contrast, the peptides from the keratan sulfate-rich and hyaluronic acid-binding regions are relatively small (47 to 12 kDa). One peptide from the hyaluronic acid-binding region appears to contain mannose-rich N-linked oligosaccharides as inferred from its observed binding by concanavalin A. A different hyaluronic acid-binding region peptide and one of the keratan sulfate-rich peptides were shown to contain disulfide bonds and therefore may be involved in contributing to the tertiary structure of the hyaluronic acid-binding region. Based on these observations, a map of the chick chondrocyte proteoglycan core protein has been constructed.
在24期鸡胚肢体芽间充质细胞培养物中，软骨细胞合成的高浮力密度蛋白聚糖的核心蛋白用溴化氰裂解，在十二烷基硫酸钠-聚丙烯酰胺平板凝胶上产生17种可分辨的肽段。在这些肽段中，10种似乎起源于富含硫酸软骨素的区域，2种似乎来源于富含硫酸角质素的区域，5种似乎来源于透明质酸结合区域。来自富含硫酸软骨素区域的肽段几乎都很大（200至64 kDa）。相比之下，来自富含硫酸角质素区域和透明质酸结合区域的肽段相对较小（47至12 kDa）。从伴刀豆球蛋白A观察到的结合情况推断，来自透明质酸结合区域的一种肽段似乎含有富含甘露糖的N-连接寡糖。已证明来自透明质酸结合区域的另一种肽段和一种富含硫酸角质素的肽段含有二硫键，因此可能参与了透明质酸结合区域三级结构的形成。基于这些观察结果，构建了鸡软骨细胞蛋白聚糖核心蛋白图谱。
