Berdoz J, Gorski J, Termijtelen A M, Dayer J M, Irlé C, Schendel D, Mach B
J Immunol. 1987 Aug 15;139(4):1336-41.
The HLA-DR subregion of the human major histocompatibility complex encodes molecules involved in the regulation of the immune response. These HLA class II molecules are transmembrane heterodimers composed of an alpha and a beta chain. The polymorphic beta chains are encoded by multiple, highly homologous loci, whereas the alpha chain is encoded by a single, nonpolymorphic locus. HLA-DR is expressed constitutively on B lymphocytes and on activated T lymphocytes. It can also be induced by interferon-gamma on most nonlymphoid cells. In a quantitative study of the expression of the individual DR beta chain loci, we have investigated: the levels of mRNA transcripts of the two functional DR beta loci (beta I and beta III) in B cells of various haplotypes; whether both beta chain loci are expressed in activated T cells and, if so, the level of expression of each; whether both loci are expressed in interferon-gamma-induced nonlymphoid cells. This analysis relied on locus-specific DR beta chain oligonucleotide probes. Expression of both the beta I and the beta III loci was observed in all cell types and in all haplotypes tested. In every case the amount of beta I mRNA was about 5 times higher than that of beta III mRNA. This indicates a controlled and coordinated regulation of the mRNA levels of these two HLA-DR loci under all conditions of major histocompatibility complex class II gene expression.
人类主要组织相容性复合体的HLA - DR亚区编码参与免疫应答调节的分子。这些HLA - II类分子是由一条α链和一条β链组成的跨膜异二聚体。多态性的β链由多个高度同源的基因座编码，而α链由单个非多态性基因座编码。HLA - DR在B淋巴细胞和活化的T淋巴细胞上组成性表达。它也可由γ干扰素在大多数非淋巴细胞上诱导表达。在一项对各个DRβ链基因座表达的定量研究中，我们研究了：不同单倍型B细胞中两个功能性DRβ基因座（βI和βIII）的mRNA转录水平；两个β链基因座在活化的T细胞中是否都表达，如果是，每个基因座的表达水平如何；两个基因座在γ干扰素诱导的非淋巴细胞中是否都表达。该分析依赖于基因座特异性的DRβ链寡核苷酸探针。在所有测试的细胞类型和所有单倍型中均观察到βI和βIII基因座的表达。在每种情况下，βI mRNA的量约比βIII mRNA高5倍。这表明在主要组织相容性复合体II类基因表达的所有条件下，这两个HLA - DR基因座的mRNA水平受到受控且协调的调节。
