Lafond-Grellety J, Hocquellet P, Serceau R
Ann Microbiol (Paris). 1978 Jul;129B(1):3-18.
A strain of Aspergillus fumigatus (Fresenius) isolated from a milk food for calves was grown on a culture medium containing added saccharose. The purpose was to study the synthesis of two recently discovered mycotoxins, fumigatin and spinulosin. The work was performed under many different conditions of temperature, pH and inoculum. These mycotoxins were measured by analytical differential pulse polarography. Correlations were observed between the growth rate of A. fumigatus and variation in pH of the medium and the formation of fumigatin, which is only possible when pH falls to less than 4.0. Fumigatin appears promptly at the beginning of the growth phase of the fungus but quickly disappears. The production of metabolite depends on limited conditions of culture. Spinulosin, very similar to fumigatin, is substituted for fumigatin in slightly different conditions. During growth, the fungus degrades both metabolites. The nature of the substitution and the reason of these modifications have not been investigated. Fumigatin and spinulosin formation is observed in both toxigenic and non-toxigenic strains.
从一种犊牛食用的乳类食品中分离出的一株烟曲霉（弗里斯）在添加了蔗糖的培养基上生长。目的是研究两种最近发现的霉菌毒素——烟曲霉素和旋孢腔菌素的合成。该研究在许多不同的温度、pH值和接种量条件下进行。这些霉菌毒素通过分析差分脉冲极谱法进行测定。观察到烟曲霉的生长速率与培养基pH值的变化以及烟曲霉素的形成之间存在相关性，而这只有在pH值降至4.0以下时才会发生。烟曲霉素在真菌生长阶段开始时迅速出现，但很快消失。代谢产物的产生取决于有限的培养条件。旋孢腔菌素与烟曲霉素非常相似，在稍有不同的条件下会替代烟曲霉素。在生长过程中，真菌会降解这两种代谢产物。替代的性质以及这些变化的原因尚未进行研究。在产毒菌株和非产毒菌株中均观察到了烟曲霉素和旋孢腔菌素的形成。
