Laboratorio de Biotecnología Ambiental, Escuela de Ingeniería en Bioquímica, Facultad de Ingeniería, Pontificia Universidad Católica de Valparaíso, 2362806, Valparaiso, Chile.
Department of Sanitary and Environmental Engineering, Federal University of Minas Gerais, Antonio Carlos Avenue 6627, Belo Horizonte, 31270-090, Brazil.
Appl Biochem Biotechnol. 2019 Nov;189(3):787-797. doi: 10.1007/s12010-019-03044-8. Epub 2019 May 23.
Anaerobic digestion of microalgal biomass for biogas production may be limited due to the cell wall resulting in an inefficient bioconversion. Enzymatic pretreatments are applied for inducing cell damage/lysis and organic matter solubilisation and this way increasing biogas production. We evaluated enzymatic pretreatments in different conditions for comparing in relation to cell wall rupture, increase of soluble material and increase in biogas production through anaerobic digestion performance in BMP assay. Chlorella sorokiniana cultures were subjected to three different enzymatic pretreatments, each under four different conditions of enzyme/substrate ratio, pH and application time. The results showed increases over 21% in biogas productions for all enzymatic pretreatments. Enzymatic pretreatment was effective at damaging microalgae cell wall, releasing organic compounds and increasing the rate and final methane yield in BMP tests. We observed a synergistic activity between the mixtures enzymes, which would depend on operational conditions used for each pretreatment.
微藻生物质的厌氧消化可能会受到细胞壁的限制，导致生物转化效率低下。酶预处理可用于诱导细胞损伤/裂解和有机物溶解，从而提高沼气产量。我们在不同条件下评估了酶预处理，以比较它们在细胞壁破裂、可溶性物质增加以及通过 BMP 分析中的厌氧消化性能提高沼气产量方面的效果。将集胞藻 sorokiniana 培养物进行了三种不同的酶预处理，每种预处理在酶/底物比、pH 值和应用时间四个不同条件下进行。结果表明，所有酶预处理的沼气产量都增加了 21%以上。酶预处理可有效破坏微藻细胞壁，释放有机化合物，并提高 BMP 试验中的速率和最终甲烷产量。我们观察到混合物酶之间存在协同作用，这取决于每种预处理使用的操作条件。
