禽流感病毒诱导的自噬增强了病毒在感染早期鸡体内的复制。
Autophagy induced by avian reovirus enhances viral replication in chickens at the early stage of infection.
机构信息
Jiangsu Co-Innovation Center for Prevention of Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, 12 East Wenhui Road, Yangzhou, 225009, Jiangsu, China.
出版信息
BMC Vet Res. 2019 May 24;15(1):173. doi: 10.1186/s12917-019-1926-5.
BACKGROUND
Avian reovirus (ARV) is an important pathogen that can cause serious disease in poultry. Though several in vitro studies revealed some molecular mechanisms that are responsible for ARV-induced autophagy, it is still largely unknown how ARV manipulates autophagy to promote its own propagation.
RESULTS
In this study, we demonstrated that ARV infection triggered autophagy in chicken tissues, evident from the enhancement of LC3-I/-II conversion and the appearance of abundant autophagosomes. Moreover, viral replication and the expression of IL-1β were coupled with the process of ARV-induced autophagy in the early stage of infection. Furthermore, regulation of autophagy affected the accumulation of LC3-II, the production of ARV and the expression of IL-1β.
CONCLUSIONS
Altogether, our data suggest that ARV induces autophagy, which benefits its replication and dissemination in chicken tissues at the early infection stage.
背景
禽呼肠孤病毒（ARV）是一种重要的病原体，可导致家禽发生严重疾病。尽管几项体外研究揭示了一些负责 ARV 诱导自噬的分子机制，但 ARV 如何操纵自噬来促进自身繁殖在很大程度上仍是未知的。
结果
在本研究中，我们证明 ARV 感染可触发鸡组织中的自噬，从 LC3-I/-II 转化增强和大量自噬体的出现可以明显看出。此外，病毒复制和 IL-1β 的表达与感染早期的 ARV 诱导自噬过程相关。此外，自噬的调控会影响 LC3-II 的积累、ARV 的产生和 IL-1β 的表达。
结论
总的来说，我们的数据表明 ARV 诱导自噬，这有利于其在感染早期在鸡组织中的复制和传播。
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