孟加拉国迈门辛地区不同区域后院鸡群中禽呼肠孤病毒的血清流行率及危险因素
Seroprevalence and risk factors of avian reovirus in backyard chickens in different areas of Mymensingh district in Bangladesh.
作者信息
Islam Md Saiful, Sabuj Abdullah Al Momen, Haque Zobayda Farzana, Pondit Amrita, Hossain Md Golzar, Saha Sukumar
机构信息
Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
出版信息
J Adv Vet Anim Res. 2020 Sep 1;7(3):546-553. doi: 10.5455/javar.2020.g452. eCollection 2020 Sep.
OBJECTIVE
The present study estimated the seroprevalence of avian reovirus (ARV) infections in backyard chickens of the Mymensingh district in Bangladesh.
MATERIALS AND METHODS
Considering several risk factors, a total of 460 serum samples were collected from backyard chickens from eight Upazilas of the Mymensingh district in Bangladesh. Blood samples were taken from the wing vein using 3-ml sterile syringes and kept at room temperature for clotting in a slanting position and then transported to the laboratory maintaining the cool chain. Subsequently, the prepared sera were harvested and stored at -20°C until used. Finally, an indirect enzyme-linked immunosorbent assay (ELISA) was performed to detect ARVspecific antibodies using a commercial ARV antibody detection ELISA test kit.
RESULTS
The results revealed high prevalence rates of ARV antibodies, with a total seroprevalence of 69.78% (321/460). Area-wise, 74.55% (82/110) seroprevalence was recorded as the highest in Mymensingh Sadar, whereas 64% (32/50) was the lowest in Gauripur Upazila. With regard to sex, female chickens showed a significantly higher ( < 0.05) seroprevalence as 90.33% (271/300) compared to male chickens 31.25% (50/160). With regard to age groups, the seroprevalence of ARV infection was 59.33% (89/150) within 2-8 weeks, 82% (205/250) within 9-16 weeks, and 45% (27/60) within 17-20 weeks, respectively. Based on hygienic conditions, the highest seroprevalence of ARV was noted in backyard chickens housed in poor conditions 80% (120/150) than good conditions 50% (40/80). Backyard chickens reared in free-ranging conditions exhibited a significantly higher seroprevalence 73.33% (220/300) of ARV antibodies compared to rearing in separate houses 63.12% (101/160). The seroprevalence of ARV was higher in crossbreeds 71.67% (43/60), brought from market 76% (38/50), and unhealthy 78.57% (55/70) backyard chickens than non-descriptive indigenous 69.5% (278/400), home-reared 69.02% (283/410), and healthy chickens 68.21% (266/390).
CONCLUSION
The high prevalence of ARV antibodies revealed in the current study indicates an extensive exposure of ARV to backyard chickens in Bangladesh that may be transmitted naturally to other chickens, ultimately leading to ominous economic effects on the poultry sector.
目的
本研究评估了孟加拉国迈门辛地区后院鸡群中禽呼肠孤病毒（ARV）感染的血清流行率。
材料与方法
考虑到多个风险因素，从孟加拉国迈门辛地区8个乡的后院鸡群中总共采集了460份血清样本。使用3毫升无菌注射器从鸡翅静脉采集血样，将其在室温下倾斜放置使其凝固，然后在保持冷链的条件下运至实验室。随后，收集制备好的血清并储存在-20°C直至使用。最后，使用商业ARV抗体检测ELISA试剂盒进行间接酶联免疫吸附测定（ELISA）以检测ARV特异性抗体。
结果
结果显示ARV抗体的流行率很高，总血清流行率为69.78%（321/460）。按地区划分，迈门辛市的血清流行率最高，为74.55%（82/110），而在戈尔布尔乡最低，为64%（32/50）。就性别而言，雌性鸡的血清流行率显著更高（<0.05），为90.33%（271/300），而雄性鸡为31.25%（50/160）。就年龄组而言，2至8周龄的鸡群中ARV感染的血清流行率为59.33%（89/150），9至16周龄为82%（205/250），17至20周龄为45%（27/60）。基于卫生条件，饲养条件差的后院鸡群中ARV的血清流行率最高，为80%（120/150），而饲养条件好的为50%（40/80）。与单独圈养的鸡群（63.12%，101/1六百）相比，自由放养条件下饲养的后院鸡群中ARV抗体的血清流行率显著更高，为73.33%（220/300）。杂交品种（71.67%，43/60）、从市场购入的鸡（76%，38/50）以及不健康的后院鸡（78.57%，55/70）中ARV的血清流行率高于非特定品种的本地鸡（69.5%，278/400）、自家饲养的鸡（69.02%，283/410）和健康鸡（68.21%，266/390）。
结论
本研究中显示的ARV抗体的高流行率表明，在孟加拉国，ARV广泛感染后院鸡群，可能会自然传播给其他鸡，最终对家禽业造成严重的经济影响。
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