PES1 在肝癌干细胞中受 CD44 通过 miR-105-5p 调控。
PES1 is regulated by CD44 in liver cancer stem cells via miR-105-5p.
机构信息
Department of Cell Biology, School of Basic Medical Sciences, Peking University Health Science Center, Haidian District, Beijing, China.
Department of Hepatobiliary Surgery II, Guangdong Provincial Research Center for Artificial Organ and Tissue Engineering, Guangzhou Clinical Research and Transformation Center for Artificial Liver, Institute of Regenerative Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
出版信息
FEBS Lett. 2019 Jul;593(14):1777-1786. doi: 10.1002/1873-3468.13459. Epub 2019 Jun 17.
Pescadillo (PES1) is a key molecule for ribosome formation in mammalian cells. In this study, human hepatoma C3A cells were reprogrammed by four transcription factors, Oct4, Sox2, Klf4 and c-Myc, into induced cancer stem cells, termed C3A-induced cancer stem cells (C3A-iCSCs). We found that PES1 was up-regulated in C3A-iCSCs and promoted cell proliferation. Moreover, the cancer stem cell marker CD44, which is located in the cytomembrane, translocated to the nucleus and was up-regulated in C3A-iCSCs. Our results suggest that CD44 has a negative effect on miR-105-5p. We found that PES1 is a direct target of, and was negatively regulated by, miR-105-5p. In summary, CD44 regulates PES1 in liver cancer stem cells via miR-105-5p to promote cell growth.
Pescadillo（PES1）是哺乳动物细胞核糖体形成的关键分子。在这项研究中，用人肝癌 C3A 细胞的四种转录因子 Oct4、Sox2、Klf4 和 c-Myc 重编程为诱导性癌症干细胞，称为 C3A 诱导性癌症干细胞（C3A-iCSCs）。我们发现 PES1 在 C3A-iCSCs 中上调，并促进细胞增殖。此外，位于细胞质膜上的癌症干细胞标志物 CD44 易位到细胞核并在 C3A-iCSCs 中上调。我们的结果表明 CD44 对 miR-105-5p 有负向作用。我们发现 PES1 是 miR-105-5p 的直接靶标，并受其负调控。总之，CD44 通过 miR-105-5p 调节肝癌干细胞中的 PES1 以促进细胞生长。
Zotero 插件