You Sohyeon, Kim Gun-Hee
Department of Health Functional Materials, Duksung Women's University , Seoul , South Korea.
Department of Food and Nutrition, Duksung Women's University , Seoul , South Korea.
Biosci Biotechnol Biochem. 2019 Oct;83(10):1893-1900. doi: 10.1080/09168451.2019.1621154. Epub 2019 May 25.
This study was undertaken to investigate the neuroprotective effect of an ethanolic extract of Mori Cortex radicis (MCR) against high glucose (HG)-induced oxidative damage in PC12 cells. Cell cytotoxicity was examined using MTT and lactate dehydrogenase assays. To examine the antioxidative effects, intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) levels and the activities of antioxidant enzymes were measured. The expressions of apoptosis-associated proteins were assessed. MCR was found to increase the viabilities of HG-induced PC12 cells and to inhibit ROS and MDA production and to promote antioxidative enzyme activities. Furthermore, MCR reduced apoptosis by upregulating p-Akt and Bcl-2/Bax ratio and reducing cytochrome c level. The main flavonoids in MCR were identified by HPLC to be kuwanon G and morusin. These results suggest the antioxidative effects of MCR protect against HG-induced oxidative stress and that MCR has potential therapeutic use for the prevention and treatment of diabetic neuro-degeneration.
本研究旨在探讨桑白皮乙醇提取物（MCR）对高糖（HG）诱导的PC12细胞氧化损伤的神经保护作用。使用MTT和乳酸脱氢酶测定法检测细胞毒性。为了检测抗氧化作用，测量了细胞内活性氧（ROS）和丙二醛（MDA）水平以及抗氧化酶的活性。评估了凋亡相关蛋白的表达。发现MCR可增加HG诱导的PC12细胞的活力，抑制ROS和MDA的产生，并促进抗氧化酶活性。此外，MCR通过上调p-Akt和Bcl-2/Bax比值并降低细胞色素c水平来减少细胞凋亡。通过高效液相色谱法鉴定出MCR中的主要黄酮类化合物为桑酮G和桑色素。这些结果表明，MCR的抗氧化作用可防止HG诱导的氧化应激，并且MCR在预防和治疗糖尿病神经退行性变方面具有潜在的治疗用途。
