Gohji K, Hamami G, Kamidono S
Hinyokika Kiyo. 1987 Mar;33(3):329-35.
The inhibitory effect of human-recombinant tumor necrosis factor (Hu-rec-TNF) alone or in combination with human recombinant gamma-interferon (rec gamma-IFN), doxorubicin (ADM) or cis-platinum (CDDP) was studied for two human renal cell carcinoma lines (KO-RCC-1 and RCC-nu-1 cells). Hu-rec-TNF inhibited the cell growth of KO-RCC-1 cells in a dose- and time-dependent manner. The inhibitory effect was seen 48 hours after the treatment with Hu-rec-TNF alone. The effect of Hu-rec-TNF was cytotoxic in our experiment. On the other hand, Hu-rec-TNF did not inhibit the cell growth of RCC-nu-1 cells. There were sensitive and resistant cells in renal cell carcinoma. In KO-RCC-1 cells, Hu-rec-TNF enhanced the inhibitory effect of rec gamma-IFN, ADM or CDDP. On the other hand, Hu-rec-TNF did not enhance the inhibitory effect of rec gamma-IFN, ADM or CDDP in RCC-nu-1 cells. In our experiment, the combined treatment with Hu-rec-TNF and rec gamma-IFN, ADM or CDDP was useful in human renal cell carcinoma which was sensitive for Hu-rec-TNF.
研究了重组人肿瘤坏死因子（Hu-rec-TNF）单独或与重组人γ干扰素（recγ-IFN）、阿霉素（ADM）或顺铂（CDDP）联合应用对两种人肾癌细胞系（KO-RCC-1和RCC-nu-1细胞）的抑制作用。Hu-rec-TNF以剂量和时间依赖性方式抑制KO-RCC-1细胞的生长。单独用Hu-rec-TNF处理48小时后可见抑制作用。在我们的实验中，Hu-rec-TNF的作用具有细胞毒性。另一方面，Hu-rec-TNF不抑制RCC-nu-1细胞的生长。肾细胞癌中存在敏感和耐药细胞。在KO-RCC-1细胞中，Hu-rec-TNF增强了recγ-IFN、ADM或CDDP的抑制作用。另一方面，Hu-rec-TNF在RCC-nu-1细胞中未增强recγ-IFN、ADM或CDDP的抑制作用。在我们的实验中，Hu-rec-TNF与recγ-IFN、ADM或CDDP联合治疗对Hu-rec-TNF敏感的人肾细胞癌有效。
