Head of Research Center, Misr International University, Cairo, Egypt; Oral Pathology Department, Faculty of Dentistry, Ain Shams University Cairo, Egypt.
Biochemistry Department, National Research Center, Cairo, Egypt; Head of Cancer Biology and Genetics Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, Dokki, 12622, Cairo, Egypt; Clinical Laboratory Department, College of Applied Medical Sciences, Taif University, Al Mutamarat Rd, Al Mathnah, At Taif 26521, Saudi Arabia.
Arch Oral Biol. 2019 Jul;103:47-54. doi: 10.1016/j.archoralbio.2019.05.011. Epub 2019 May 14.
This study aims to investigate the apoptotic effect of Doxorubicin and its nano-formulated form (Doxil) on oral squamous cell carcinoma CAL-27 cells.
Cell viability using MTT assay, mode of cell death using fluorescence analysis, expression of the apoptotic marker caspase-3 using indirect ELISA technique and expression of C-Myc gene using reverse transcriptase and real time PCR.
Doxil treatment resulted in a higher percentage of apoptotic cells than doxorubicin treatment, while doxorubicin treatment resulted in a higher percentage of necrotic cells than Doxil treatment. Doxil-treated cells exhibited 3.38-fold higher caspase-3 levels than control cells, while doxorubicin significantly increased caspase-3 levels by 2.72-fold. The percentage of C-Myc mRNA inhibition was 27% in doxorubicin-treated cells and 41% in Doxil-treated cells.
Doxil exerted a higher apoptotic effect on CAL-27 cells compared to doxorubicin. It showed a higher increase in capase-3 level than doxorubicin and also exerted a more percentage of C-Myc mRNA inhibition.
本研究旨在探讨阿霉素及其纳米制剂（多柔比星脂质体）对口腔鳞状细胞癌细胞 CAL-27 的凋亡作用。
采用 MTT 法检测细胞活力，荧光分析检测细胞死亡方式，间接 ELISA 技术检测凋亡标志物 caspase-3 的表达，逆转录和实时 PCR 检测 C-Myc 基因的表达。
与阿霉素处理相比，多柔比星脂质体处理导致更高比例的凋亡细胞，而阿霉素处理导致更高比例的坏死细胞。多柔比星脂质体处理的细胞中 caspase-3 水平比对照细胞高 3.38 倍，而阿霉素显著增加了 caspase-3 水平 2.72 倍。阿霉素处理的细胞中 C-Myc mRNA 的抑制率为 27%，多柔比星脂质体处理的细胞中为 41%。
与阿霉素相比，多柔比星脂质体对 CAL-27 细胞的凋亡作用更强。它显示出比阿霉素更高的 caspase-3 水平增加，并且还发挥了更高比例的 C-Myc mRNA 抑制作用。
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