Guinea-pig lymphotoxin resistant L-cell sublines.

Guinea-pig lymphotoxin (GLT)-resistant L-cell sublines were isolated, and the altered properties were characterized. Two of them (No. 1 and No. 2 cells) showed the lowest capacity to absorb GLT and substantially reduced ability to bind labelled Ricinus communis agglutinin (RcA). Both the cell-surface proteins and glycoproteins of No. 2 cells were found to be quite different from those of parental L cells. The glycoprotein of molecular weight 140,000, which specifically bound to RcA-Sepharose, was missing in the No. 2 cells extract. These results indicate that the glycoprotein of molecular weight 140,000 is the most probable candidate for the receptor molecule for GLT, and that No. 2 cells and probably No. 1 cells are GLT receptor-deficient cells.