Shaw R W, Ndukwe G, Imoedemhe D A, Bernard A, Burford G, Bentick B
Br J Obstet Gynaecol. 1987 Jul;94(7):682-6. doi: 10.1111/j.1471-0528.1987.tb03175.x.
Pituitary desensitization was induced in 13 normally menstruating women by administration of an LHRH agonist, Buserelin, intranasally, 100 micrograms at intervals of 4 h, for a period of 14 days starting on day 22 of the menstrual cycle. Whilst continuing with Buserelin, multiple follicular development was induced by administration of purified FSH (Metrodin) for between 8 and 15 days at a dose of 150 i.u. daily. In one patient no follicular growth was induced after 14 days treatment. In the remaining subjects between 3 and 11 follicles with mean diameter greater than 16 mm were stimulated and between 2 and 10 mature oocytes were recovered laparoscopically for IVF, 34 h after hCG administration. Eight patients underwent embryo transfer and four conceived.
对13名月经正常的女性进行垂体脱敏，从月经周期的第22天开始，经鼻内给予促黄体生成素释放激素（LHRH）激动剂布舍瑞林，每次100微克，间隔4小时，持续14天。在继续使用布舍瑞林的同时，通过每日皮下注射150国际单位的纯化促卵泡激素（Metrodin），诱导多个卵泡发育，持续8至15天。1例患者在治疗14天后未诱导出卵泡生长。其余受试者中，平均直径大于16毫米的卵泡有3至11个受到刺激，并在注射人绒毛膜促性腺激素（hCG）34小时后，通过腹腔镜回收2至10个成熟卵母细胞用于体外受精（IVF）。8例患者接受了胚胎移植，4例受孕。
