Centro de Biotecnologia, Instituto Butantan, Av. Vital Brasil, 1500, 05503-900 São Paulo, SP, Brazil; Instituto Gonçalo Moniz, Fundação Oswaldo Cruz (FIOCRUZ), Rua Waldemar Falcão, Salvador, Bahia, Brazil.
Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, SY23 3FG Aberystwyth, UK.
Int J Parasitol. 2019 Jul;49(8):593-599. doi: 10.1016/j.ijpara.2019.03.003. Epub 2019 May 25.
Schistosoma mansoni venom allergen-like proteins (SmVALs) are part of a diverse protein superfamily partitioned into two groups (group 1 and group 2). Phylogenetic analyses of group 1 SmVALs revealed that members could be segregated into subclades (A-D); these subclades share similar gene expression patterns across the parasite lifecycle and immunological cross-reactivity. Furthermore, whole-mount in situ hybridization demonstrated that the phylogenetically, transcriptionally and immunologically-related SmVAL4, 10, 18 and 19 (subclade C) were all localized to the pre-acetabular glands of immature cercariae. Our results suggest that SmVAL group 1 phylogenetic relationships, stage-specific transcriptional profiles and tissue localization are predictive of immunological cross-reactivity.
曼氏血吸虫毒液过敏原样蛋白（SmVALs）是一个多样化蛋白超家族的一部分，可分为两组（第 1 组和第 2 组）。对第 1 组 SmVALs 的系统发育分析表明，成员可分为亚群（A-D）；这些亚群在整个寄生虫生命周期和免疫交叉反应中具有相似的基因表达模式。此外，整体原位杂交表明，在系统发育、转录和免疫上相关的 SmVAL4、10、18 和 19（C 亚群）均定位于未成熟尾蚴的前附腺。我们的结果表明，SmVAL 第 1 组的系统发育关系、阶段特异性转录谱和组织定位可预测免疫交叉反应性。
