Felmingham D, Robbins M J, Marais R, Ridgway G L, Gruneberg R N
Drugs Exp Clin Res. 1987;13(4):195-9.
The in vitro activity of erythromycin and RU-28965 (a novel macrolide antimicrobial with improved pharmacokinetics) was determined against a variety of anaerobic bacteria in anaerobic atmospheres with and without added carbon dioxide. Minimum inhibitory concentrations (MIC) were determined using an antimicrobial incorporation technique in Wilkins-Chalgren agar (Oxoid, UK) containing saponinlysed horse blood to a final concentration of 10%. The inoculum used was approximately 10(4) colony forming units (cfu) contained in 10 microliters Wilkins-Chalgren broth, which was applied to the surface of the agar plates using a multipoint inoculator. Following inoculation, plates were incubated for 48 h at 37 degrees C in an anaerobic atmosphere containing 10% carbon dioxide or in hydrogen alone. The MIC of each antimicrobial for each organism examined was determined as the lowest concentration of the antimicrobial which completely inhibited growth of the inoculum. The minimum concentrations required to inhibit the growth of 50% (MIC50) and 90% (MIC90) of the bacteria examined were also determined. The MICs of erythromycin and RU-28965 for isolates of the Bacteroides fragilis group, B. bivius and Fusobacterium spp. were generally 10-100 times greater when determined in the presence of carbon dioxide than when determined in hydrogen alone. The MICs of erythromycin and RU-28965 for B. melaninogenicus, Peptococcus spp., Peptostreptococcus spp., Clostridium perfringens, Cl. difficile and Propionibacterium acnes were less affected by the presence of carbon dioxide.
在有和没有添加二氧化碳的厌氧环境中，测定了红霉素和RU - 28965（一种具有改善药代动力学的新型大环内酯类抗菌药物）对多种厌氧菌的体外活性。使用抗菌药物掺入技术在含有终浓度为10%皂素裂解马血的威尔金斯 - 查尔格伦琼脂（英国奥克托德公司）中测定最低抑菌浓度（MIC）。所用接种物为10微升威尔金斯 - 查尔格伦肉汤中约含10(4)个菌落形成单位（cfu），使用多点接种器将其接种到琼脂平板表面。接种后，平板在含有10%二氧化碳的厌氧环境或仅在氢气环境中于37℃孵育48小时。每种抗菌药物对每种受试微生物的MIC被确定为完全抑制接种物生长的抗菌药物的最低浓度。还测定了抑制所检测细菌50%（MIC50）和90%（MIC90）生长所需的最低浓度。当在有二氧化碳存在的情况下测定时，红霉素和RU - 28965对脆弱拟杆菌属、双栖拟杆菌和梭杆菌属菌株的MIC通常比仅在氢气环境中测定时大10 - 100倍。红霉素和RU - 28965对产黑色素拟杆菌、消化球菌属、消化链球菌属、产气荚膜梭菌、艰难梭菌和痤疮丙酸杆菌的MIC受二氧化碳存在的影响较小。
