Robbins M J, Marais R, Felmingham D, Ridgway G L, Grüneberg R N
Department of Clinical Microbiology, University College Hospital, London, UK.
Drugs Exp Clin Res. 1987;13(9):551-4.
The in vitro activity of vancomycin and teicoplanin, a new glycopeptide antimicrobial, was determined against a total of 286 anaerobic bacteria including Bacteroides fragilis group (100), B. melaninogenicus (21), B. bivius (16), Fusobacterium spp. (15), Peptococcus spp. (20), Peptostreptococcus spp. (21), Clostridium perfringens (23), C. difficile (41) and Propionibacterium acnes (29). Minimum inhibitory concentrations (MIC) were determined using an antimicrobial incorporation technique in Wilkins-Chalgren agar approximately 10(4) colony forming units (cfu) contained in 10 microliters Wilkins-Chalgren broth, which was applied to the surface of the agar plates using a multipoint inoculator. Following inoculation, plates were incubated for 48 h at 37 degrees C in an anaerobic atmosphere. Both vancomycin and teicoplanin were highly active against all the Gram-positive anaerobic bacteria examined, 90% of all isolates being inhibited by 0.5 micrograms/ml of either antimicrobial. Isolates of B. fragilis group and Fusobacterium spp. were resistant to vancomycin (MIC90 64 micrograms/ml) and teicoplanin (MIC90 128 micrograms/ml). Unexpectedly, isolates of B. melaninogenicus and B. bivius which were resistant to vancomycin (MIC90 64 and 128 micrograms/ml respectively) were sensitive to teicoplanin (MIC90 2 and 2 micrograms/ml respectively).
测定了万古霉素和一种新型糖肽类抗菌药物替考拉宁对总共286株厌氧菌的体外活性，这些厌氧菌包括脆弱拟杆菌群（100株）、产黑色素拟杆菌（21株）、双路拟杆菌（16株）、梭杆菌属（15株）、消化球菌属（20株）、消化链球菌属（21株）、产气荚膜梭菌（23株）、艰难梭菌（41株）和痤疮丙酸杆菌（29株）。采用抗菌药物掺入技术在威尔金斯 - 查尔格伦琼脂中测定最低抑菌浓度（MIC），将10微升威尔金斯 - 查尔格伦肉汤中含有的约10⁴菌落形成单位（cfu），用多点接种器接种到琼脂平板表面。接种后，平板在37℃厌氧环境中孵育48小时。万古霉素和替考拉宁对所有检测的革兰氏阳性厌氧菌均具有高活性，所有分离株中有90%被0.5微克/毫升的任何一种抗菌药物抑制。脆弱拟杆菌群和梭杆菌属的分离株对万古霉素（MIC90为64微克/毫升）和替考拉宁（MIC90为128微克/毫升）耐药。出乎意料的是，对万古霉素耐药（MIC90分别为64和128微克/毫升）的产黑色素拟杆菌和双路拟杆菌分离株对替考拉宁敏感（MIC90分别为2和2微克/毫升）。
