De Stefano V, Leone G, De Martini D, Di Donfrancesco A, Accorrà F, Bizzi B
Haemostasis. 1987;17(4):195-200. doi: 10.1159/000215743.
Antithrombin III (AT III) functional levels are much lower in serum than in plasma; during oral anticoagulation this difference is reduced. Plasma and serum of 172 patients taking vitamin K antagonists were tested for AT III antigen and both AT III heparin cofactor and anti-Xa heparin cofactor. Crossed immunoelectrophoresis of AT III on heparin-agarose was also carried out in plasma and serum. The patients were divided into four groups: (1) international normalized ratio (INR) 9.3-4.1, n = 25; (2) INR 4.0-2.5, n = 73; (3) INR 2.4-2.0, n = 40, and (4) INR 1.9-1.5, n = 34. 66 healthy subjects were used as controls. Plasma levels of AT III antigen, AT III heparin cofactor, and anti-Xa heparin cofactor were the same in all groups. In all groups all serum AT III parameters were higher than in controls; crossed immunoelectrophoresis of AT III on heparin-agarose indicated that this finding was due to a lower formation of complexed AT III in serum. AT III heparin cofactor serum values were the same whatever the INR over a large range (9.3-1.5); the highest anti-Xa heparin cofactor serum levels were noted in the groups treated more intensely (groups 1 and 2).
抗凝血酶III（AT III）的功能水平在血清中比在血浆中低得多；在口服抗凝治疗期间，这种差异会减小。对172名服用维生素K拮抗剂的患者的血浆和血清进行了AT III抗原、AT III肝素辅因子和抗Xa肝素辅因子的检测。还对血浆和血清中的AT III在肝素琼脂糖上进行了交叉免疫电泳。患者被分为四组：（1）国际标准化比值（INR）9.3 - 4.1，n = 25；（2）INR 4.0 - 2.5，n = 73；（3）INR 2.4 - 2.0，n = 40，以及（4）INR 1.9 - 1.5，n = 34。66名健康受试者作为对照。所有组中AT III抗原、AT III肝素辅因子和抗Xa肝素辅因子的血浆水平相同。在所有组中，所有血清AT III参数均高于对照组；AT III在肝素琼脂糖上的交叉免疫电泳表明，这一发现是由于血清中结合型AT III的形成较少。无论INR在较大范围内（9.3 - 1.5）如何，AT III肝素辅因子的血清值均相同；在强化治疗组（第1组和第2组）中观察到最高的抗Xa肝素辅因子血清水平。
