Swoboda R, Wecker E, Schimpl A
Immunobiology. 1987 May;174(3):300-12. doi: 10.1016/S0171-2985(87)80005-0.
In mitogen-stimulated mouse spleen cells, the IL 2 gene is only transiently expressed with maximal mRNA steady state levels between 6-14 h post stimulation with Concanavalin A (Con A). This is also reflected by the kinetics of IL 2 release into culture supernatants. Con A-stimulated L3T4+ and Lyt2+ T cell subpopulations express the IL 2 gene and produce IL 2 similarly. The half-life of IL 2 mRNA is only 30 min, but can be prolonged significantly by cycloheximide. At later post stimulation times IL 2 gene transcription is reduced, as indicated by the reduced effect of cycloheximide. IL 2 gene expression is not influenced by added IL 2 or IFN-gamma.
在丝裂原刺激的小鼠脾细胞中，白细胞介素2（IL 2）基因仅短暂表达，在用刀豆蛋白A（Con A）刺激后6 - 14小时达到最大mRNA稳态水平。这也反映在IL 2释放到培养上清液中的动力学上。Con A刺激的L3T4 +和Lyt2 + T细胞亚群表达IL 2基因并类似地产生IL 2。IL 2 mRNA的半衰期仅为30分钟，但可被放线菌酮显著延长。在刺激后的后期，如放线菌酮作用减弱所示，IL 2基因转录减少。IL 2基因表达不受添加的IL 2或干扰素γ的影响。
