Drummond I A, Lee A S, Resendez E, Steinhardt R A
J Biol Chem. 1987 Sep 15;262(26):12801-5.
Animal cells respond to calcium ionophore (A23187) treatment with the coordinate induction of a set of genes encoding proteins identical to the glucose-regulated proteins (GRPs). By monitoring the intracellular free calcium with the fluorescent indicator fura-2 while employing both intracellular and extracellular calcium buffers, we demonstrated that A23187 can induce the GRP94 and GRP78 genes without an increase in cytoplasmic calcium ([Ca2+]i). Induction of GRP mRNA during glucose starvation was also independent of [Ca2+]i. Instead, gene induction by A23187 was closely correlated with the depletion of intracellular calcium stores. We conclude that perturbations of sequestered calcium ions by A23187 can serve as a stimulus for gene expression.
动物细胞在受到钙离子载体（A23187）处理时，会协同诱导一组编码与葡萄糖调节蛋白（GRP）相同蛋白质的基因。通过使用荧光指示剂fura-2监测细胞内游离钙，并同时使用细胞内和细胞外钙缓冲剂，我们证明A23187可以诱导GRP94和GRP78基因，而不会使细胞质钙（[Ca2+]i）增加。在葡萄糖饥饿期间GRP mRNA的诱导也与[Ca2+]i无关。相反，A23187对基因的诱导与细胞内钙储存的耗尽密切相关。我们得出结论，A23187对螯合钙离子的扰动可以作为基因表达的刺激因素。
