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一种用于淋病奈瑟菌运输、富集及培养检测的新技术。

A new technic for transport, enrichment, and cultural detection of Neisseria gonorrhoeae.

作者信息

Gernand K, Sönnichsen N, Kölzsch J

出版信息

J Am Acad Dermatol. 1987 Aug;17(2 Pt 1):197-200. doi: 10.1016/s0190-9622(87)70190-x.

Abstract

This article describes a new transport and enrichment technic that improves growth conditions of Neisseria gonorrhoeae and that is particularly suitable for use in dermatology clinics with no bacteriology facilities. The deep-prick culture makes use of a simple and proven selective medium, which is placed in small plastic or glass vessels. After inoculation deep into the agar medium, the gonococci themselves create the required microaerophilic environment. Inoculated smear material can therefore be transported without loss. Our experience, which has lasted for more than 3 years and which has included more than 4000 patients, showed that in 98% to 99% of cases of suspected gonorrhea, gonococci can be detected with a contamination rate of less than 3%.

摘要

本文介绍了一种新的运输和富集技术,该技术改善了淋病奈瑟菌的生长条件,特别适用于没有细菌学设施的皮肤科诊所。深刺培养法使用一种简单且经过验证的选择性培养基,将其置于小型塑料或玻璃容器中。将样本深接种到琼脂培养基中后,淋球菌自身会创造所需的微需氧环境。因此,接种的涂片材料可以无损运输。我们持续了3年多、涵盖4000多名患者的经验表明,在98%至99%的疑似淋病病例中,可以检测到淋球菌,污染率低于3%。

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