克隆的双生病毒DNA的感染性：一项评估。
Infectivity of cloned begomoviral DNAs: an appraisal.
作者信息
Kushawaha Akhilesh Kumar, Dasgupta Indranil
机构信息
Department of Plant Molecular Biology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110021 India.
出版信息
Virusdisease. 2019 Mar;30(1):13-21. doi: 10.1007/s13337-018-0453-5. Epub 2018 May 3.
Abstract
Infectivity of cloned begomoviral DNAs is an important criterion to establish the etiology of the disease it causes, to study viral gene functions and host-virus interactions. Three main methods have been employed to study infectivity; mechanical inoculation with cloned viral DNA using abrasives, Agrobacterium-mediated inoculation (agroinoculation) of cloned viral DNA and bombardment using microprojectiles coated with cloned viral DNA (biolistics). Each method has its own advantages and disadvantages and the adoption of one over the other for demonstrating infectivity depends on various factors. This review compares the various features associated with the above three methods.
摘要
克隆的双生病毒DNA的感染性是确定其所引起疾病的病因、研究病毒基因功能以及宿主-病毒相互作用的重要标准。研究感染性主要采用了三种方法：使用研磨剂对克隆的病毒DNA进行机械接种、对克隆的病毒DNA进行农杆菌介导接种（农杆菌接种法）以及使用包被有克隆病毒DNA的微粒进行轰击（生物弹道学）。每种方法都有其自身的优缺点，而在证明感染性时选择使用哪种方法取决于多种因素。本综述比较了与上述三种方法相关的各种特征。
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