College of Pharmaceutical Sciences, Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Hebei University, 180 WuSi Road, Lianchi District, Baoding 071002, China.
Food Funct. 2019 Jun 19;10(6):3514-3534. doi: 10.1039/c9fo00153k.
This study was conducted to investigate the beneficial effects and possible mechanism of action of mangiferin (MF) in alcohol hepatitis (AH) rats. Building on our previous study, the damage-associated molecular patterns (DAMPs), lipid metabolic disorder and mitochondrial dysfunction were investigated. MF effectively regulated the abnormal liver function, the levels of alcohol, FFAs and metal elements in serum. More importantly, MF improved the expression levels of mRNA and protein of PPAR-γ, OPA-1, Cav-1, EB1, NF-κB p65, NLRP3, Cas-1 and IL-1β, and decreased the positive protein expression rates of HSP90, HMGB1, SYK, CCL20, C-CAS-3, C-PARP and STARD1. Additionally, MF decreased the levels of fumarate, cAMP, xanthurenic acid and d-glucurone-6,3-lactone, and increased the levels of hippuric acid and phenylacetylglycine, and then adjusted the changes of phenylalanine metabolism, TCA cycle and ascorbate and aldarate metabolic pathways. The above results suggested that MF can effectively prevent AH by modulating specific AH-associated genes, potential biomarkers and metabolic pathways in AH rats, etc.
本研究旨在探讨芒果苷（MF）对酒精性肝炎（AH）大鼠的有益作用及可能的作用机制。在我们之前的研究基础上，进一步探讨了损伤相关分子模式（DAMPs）、脂质代谢紊乱和线粒体功能障碍。MF 能有效调节异常肝功能，血清中酒精、FFAs 和金属元素水平。更重要的是，MF 改善了 PPAR-γ、OPA-1、Cav-1、EB1、NF-κB p65、NLRP3、Cas-1 和 IL-1β 的 mRNA 和蛋白表达水平，降低了 HSP90、HMGB1、SYK、CCL20、C-CAS-3、C-PARP 和 STARD1 的阳性蛋白表达率。此外，MF 降低了延胡索酸、cAMP、黄嘌呤酸和 D-葡糖醛酸-6,3-内酯的水平，增加了马尿酸和苯乙酰甘氨酸的水平，从而调节了苯丙氨酸代谢、三羧酸循环以及抗坏血酸和醛糖酸盐代谢途径的变化。上述结果表明，MF 通过调节 AH 大鼠中特定的 AH 相关基因、潜在生物标志物和代谢途径等，可有效预防 AH。
