Farfour Eric, Henry Amandine, Razillard Anthony, Cardot Emilie, Limousin Lucie, Cahen Pierre, Jolly Emilie, Vasse Marc, Mathonnet Damien
Service de biologie clinique, Hôpital Foch, Suresnes, France.
Service de biologie clinique, Hôpital Foch, Suresnes, France, Service de biologie, Hôpital A. Mignot, Le Chesnay, France.
Ann Biol Clin (Paris). 2019 Jun 1;77(3):350-352. doi: 10.1684/abc.2019.1446.
CHROMmagar Orientation media (Becton Dickinson) was developed and validated for the culture of urinary samples. It allows a direct identification of E. coli colonies without additional tests. As CHROMmagar Orientation media is superior to non-chromogenic media for the distinction of enterobacterial colonies, it is used for the inoculation of a large variety of samples in clinical laboratories. Direct identification of E. coli colonies cultured from these samples is not validated by the manufacturer. The difference in microbial ecology and the nature of the sample may impact CHROMagar Orientation performances for this use. We evaluated these media for the direct identification of E. coli colonies from 410 samples (excluding urine). Its sensitivity of 99% allows a direct identification of E. coli colonies cultured from a wide variety of samples. On-site testing using a large number of representative samples, allows laboratories to assess agar media performance and adapt their uses. Suppliers who are aware of frequent and non-recommended use of their culture media should perform tests and if conclusive, adapt their technical instructions.
CHROMmagar定向培养基（碧迪医疗）专为尿液样本培养而研发并经过验证。它无需额外检测就能直接鉴定出大肠杆菌菌落。由于CHROMmagar定向培养基在区分肠杆菌菌落方面优于非显色培养基，因此在临床实验室中用于接种多种样本。制造商未对从这些样本中培养出的大肠杆菌菌落进行直接鉴定的验证。微生物生态的差异和样本的性质可能会影响CHROMagar定向培养基在此用途上的性能。我们评估了这些培养基用于从410份样本（不包括尿液）中直接鉴定大肠杆菌菌落的情况。其99%的灵敏度能够直接鉴定出从多种样本中培养出的大肠杆菌菌落。通过使用大量具有代表性的样本进行现场测试，实验室可以评估琼脂培养基的性能并调整其用途。知晓其培养基经常被不当使用的供应商应进行测试，若结果确凿，应调整其技术说明书。
