用于评估水不溶性化合物针对生物相关自由基的抗氧化活性的脂质体整合方法：avarol 的实例。

Liposomal integration method for assessing antioxidative activity of water insoluble compounds towards biologically relevant free radicals: example of avarol.

机构信息

Faculty of Physical Chemistry, University of Belgrade, Belgrade, Serbia.

Department of Life Sciences, Institute for Multidisciplinary Research - IMSI, University of Belgrade, Belgrade, Serbia.

出版信息

J Liposome Res. 2020 Sep;30(3):218-226. doi: 10.1080/08982104.2019.1625378. Epub 2019 Jun 14.

DOI:10.1080/08982104.2019.1625378

PMID:31146614

Abstract

The liposomal integration method, in conjunction with electron paramagnetic resonance (EPR) spectroscopy, has been presented for the investigation of antioxidant activity of selected water-insoluble compound towards biologically relevant free radicals. This method was applied to avarol, a sesquiterpenoid hydroquinone isolated from the marine sponge . The antioxidant activity of water-insoluble avarol towards OH, O and NO radicals was attained by its incorporation into the DPPC liposomes bilayer, and towards ascorbyl radicals in the organic solvent. Avarol's activity towards OH, O, NO and ascorbyl radicals was 86.2%, 50.9%, 23.6% and 61.8%, respectively, showing its significant radical scavenging potential.

摘要

脂质体整合方法结合电子顺磁共振（EPR）光谱学，已被用于研究选定的疏水性化合物对生物相关自由基的抗氧化活性。该方法应用于avarol，avarol 是一种从海洋海绵中分离出的倍半萜类氢醌。疏水性 avarol 通过掺入 DPPC 脂质体双层结构中来实现对 OH、O 和 NO 自由基的抗氧化活性，并在有机溶剂中实现对抗坏血基自由基的抗氧化活性。avarol 对 OH、O、NO 和抗坏血基自由基的活性分别为 86.2%、50.9%、23.6%和 61.8%，显示出其显著的自由基清除潜力。