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富含酚类物质的强抗氧化水果?25个草莓品种的比较研究。

Rich in Phenolics-Strong Antioxidant Fruit? Comparative Study of 25 Strawberry Cultivars.

作者信息

Milosavljević Dragica M, Maksimović Vuk M, Milivojević Jasminka M, Nakarada Đura J, Mojović Miloš D, Dragišić Maksimović Jelena J

机构信息

Department of Life Sciences, Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, 11030 Belgrade, Serbia.

Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade, Serbia.

出版信息

Plants (Basel). 2022 Dec 17;11(24):3566. doi: 10.3390/plants11243566.

Abstract

Phenolic compounds of 25 newly introduced strawberry cultivars were profiled using spectrophotometry, electron paramagnetic resonance (EPR) spectroscopy, and high-performance liquid chromatography-mass spectrometry. Total phenolic and anthocyanin content (TPC and TACY, respectively), as well as vitamin C, and concentrations of individual phenolic compounds in fruits were evaluated to identify the most promising cultivars according to their phenolic profile. The highest values of TPC, TACY, and vitamin C were recorded in 'Premy' (1.53 mg eq GA g FW), 'Sandra' (30.60 mg eq Pg-3-g 100 g FW), and 'Laetitia' (56.32 mg 100 g FW), respectively. The DPPH and •OH radicals scavenging activity of fruit methanolic extracts was estimated using EPR spectroscopy. All cultivars are almost uniformly effective in the scavenging of •OH radical, while 'Tea', 'Premy', and 'Joly' were marked as highly potent cultivars (over 70%) in terms of DPPH-antiradical activity. Specific peroxidase activities were the highest in 'Garda', 'Federica', and 'Rumba' (0.11, 0.08, and 0.06 U mg prot, respectively). 'Laetitia', 'Joly', 'Arianna', 'Tea', and 'Mila' cultivars were distinguished from others as the richest concerning almost all flavonoids and phenolic acids, including some other parameters of bioactivity. These cultivars could be recommended to consumers as functional fruit foods.

摘要

采用分光光度法、电子顺磁共振(EPR)光谱法和高效液相色谱-质谱联用技术,对25个新引进草莓品种的酚类化合物进行了分析。评估了果实中总酚和花青素含量(分别为TPC和TACY)、维生素C以及各酚类化合物的浓度,以便根据其酚类特征确定最有前景的品种。“Premy”(1.53毫克当量没食子酸/克鲜重)、“Sandra”(30.60毫克当量矢车菊素-3-葡萄糖苷/100克鲜重)和“Laetitia”(56.32毫克/100克鲜重)的TPC、TACY和维生素C含量最高。利用EPR光谱法评估了果实甲醇提取物对DPPH和•OH自由基的清除活性。所有品种对•OH自由基的清除效果几乎一致,而“Tea”、“Premy”和“Joly”在DPPH抗自由基活性方面被标记为高效品种(超过70%)。“Garda”、“Federica”和“Rumba”的过氧化物酶比活性最高(分别为0.11、0.08和0.06单位/毫克蛋白)。“Laetitia”、“Joly”、“Arianna”、“Tea”和“Mila”品种在几乎所有黄酮类化合物和酚酸方面以及其他一些生物活性参数方面均比其他品种丰富。这些品种可作为功能性水果食品推荐给消费者。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b13/9784063/89f7304bebe8/plants-11-03566-g001.jpg

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