Albrechtová Jana, Kubínová Zuzana, Soukup Aleš, Janáček Jiří
Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic.
Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic.
Methods Mol Biol. 2019;1992:109-119. doi: 10.1007/978-1-4939-9469-4_7.
This chapter gives examples of basic procedures of quantification of plant structures with use of image analysis, which are commonly employed to describe differences among experimental treatments or phenotypes of plant material. Tasks are demonstrated with the use of ImageJ, a widely used public domain Java image processing program. Principles of sampling design based on systematic uniform random sampling for quantitative studies of anatomical parameters are given to obtain their unbiased estimations and simplified "rules of thumb" are presented. The basic procedures mentioned in the text are: (1) sampling, (2) calibration, (3) manual length measurement, (4) leaf surface area measurement, (5) estimation of particle density demonstrated on an example of stomatal density, and (6) analysis of epidermal cell shape.
本章给出了使用图像分析对植物结构进行量化的基本程序示例，这些程序通常用于描述实验处理之间的差异或植物材料的表型。通过使用ImageJ（一个广泛使用的公共领域Java图像处理程序）来演示各项任务。给出了基于系统均匀随机抽样的抽样设计原则，用于解剖参数的定量研究以获得无偏估计，并提出了简化的“经验法则”。文中提到的基本程序有：（1）抽样，（2）校准，（3）手动长度测量，（4）叶表面积测量，（5）以气孔密度为例演示颗粒密度估计，以及（6）表皮细胞形状分析。
