Graham S M, Arinze I J
Res Commun Chem Pathol Pharmacol. 1987 Jun;56(3):301-20.
Scatchard plots of the binding of [3H]Gpp(NH)p to rabbit and guinea pig liver plasma membranes were curvilinear, and could be resolved by computer-assisted non-linear regression analysis into two sites which exhibit high and low affinities. The two sites appear to be of equal capacities. The Kd values for binding to rabbit liver membranes were 5.6 +/- 0.5 nM and 133 +/- 20 nM for the high and low affinity sites, respectively. The Kd values for binding to similar sites on guinea pig liver membranes were 7.6 +/- 0.9 nM and 289 +/- 75 nM. In addition to Ns and Ni which have been identified in liver membranes, one or more of the other GTP-binding proteins may contribute to the observed binding.
[3H]Gpp(NH)p与兔和豚鼠肝细胞膜结合的Scatchard图呈曲线状，可通过计算机辅助非线性回归分析解析为具有高亲和力和低亲和力的两个位点。这两个位点的容量似乎相等。与兔肝细胞膜结合的高亲和力和低亲和力位点的Kd值分别为5.6±0.5 nM和133±20 nM。与豚鼠肝细胞膜上类似位点结合的Kd值为7.6±0.9 nM和289±75 nM。除了在肝细胞膜中已鉴定出的Ns和Ni外，其他一种或多种GTP结合蛋白可能也参与了观察到的结合。
