Yildirim A B, Ozdamar S, Kaymak E
University of Kahramanmaras Sutcu Imam, Dept. of Histology Embriyology, Kahramanmaraş, Kayseri, Turkey.
University of Erciyes, Dept. of Histology Embryology, Kayseri, Turkey.
Acta Endocrinol (Buchar). 2017 Jan-Mar;13(1):7-16. doi: 10.4183/aeb.2017.7.
Abnormal thyroid function affect spermato-genesis and male infertility. For men, the aromatase deficiency can cause infertility. In this study, the aim is to investigate the effect of maternal hypothyroidism on offspring testicular morphology and cytochrome-P450-aromatase (P450arom) immunoreactivity.
Eighteen Wistar albino pregnant rats were divided into three groups, namely A, B and K groups. Hypothyroidism was induced by adding 0.01% of propyl thiouracil (PTU) in drinking water. Hypothyroid mothers, group A: given PTU for 21 days during pregnancy, group B: given PTU for 21 days prior to pregnancy; control mothers, group K, given only water. Hypothyroid and control group mothers' pups at postnatal day (PND) 15 and 60 were sacrificed. We determined immunoreactivity intensity of P450arom and mRNA levels by RT-PCR performed in the testis tissues. ELISA method was used for thyroid function tests for T3, T4 and TSH. Structure of seminiferous tubule was evaluated by hematoxylin-eosin staining.
It was seen that the aromatase expression in 15-day-old maternal hypothyroid groups was similar to the one in the control group while there was a decline in the aromatase expression of 60-day-old groups. As for mRNA, it was determined that it had a tendency to increase over time in all groups but this increase was not significant. The tubule diameter and Johnsen's Testicular Biopsy Score diminished in all hypothyroid groups in comparison to the control group.
The changes that occur in the early period of testis development due to maternal hypothyroidism negatively affect testis development in the next stages of life. This situation leads to a decline in aromatase expression in the following years.
甲状腺功能异常会影响精子生成及男性不育。对男性而言，芳香化酶缺乏可导致不育。本研究旨在探讨母体甲状腺功能减退对后代睾丸形态及细胞色素P450芳香化酶（P450arom）免疫反应性的影响。
将18只Wistar白化病孕鼠分为三组，即A组、B组和K组。通过在饮用水中添加0.01%的丙基硫氧嘧啶（PTU）诱导甲状腺功能减退。甲状腺功能减退的母亲，A组：孕期给予PTU 21天，B组：孕前给予PTU 21天；对照组母亲，K组，仅给予水。在出生后第15天和60天处死甲状腺功能减退组和对照组母亲的幼崽。我们通过在睾丸组织中进行的逆转录聚合酶链反应（RT-PCR）测定P450arom的免疫反应强度和mRNA水平。采用酶联免疫吸附测定（ELISA）法检测T3、T4和促甲状腺激素（TSH）的甲状腺功能。通过苏木精-伊红染色评估生精小管的结构。
可见，15日龄母体甲状腺功能减退组的芳香化酶表达与对照组相似，而60日龄组的芳香化酶表达有所下降。至于mRNA，确定所有组中其随时间有增加的趋势，但这种增加不显著。与对照组相比，所有甲状腺功能减退组的小管直径和约翰森睾丸活检评分均降低。
母体甲状腺功能减退在睾丸发育早期发生的变化对生命后期的睾丸发育产生负面影响。这种情况导致随后几年芳香化酶表达下降。
