Yin Yong-Gen, Ishii Satomi, Suzui Nobuo, Igura Masato, Kurita Keisuke, Miyoshi Yuta, Nagasawa Naotsugu, Taguchi Mitsumasa, Kawachi Naoki
Takasaki Advanced Radiation Research Institute, National Institutes for Quantum and Radiological Science and Technology (QST), Takasaki, Gunma, 370-1292, Japan.
Takasaki Advanced Radiation Research Institute, National Institutes for Quantum and Radiological Science and Technology (QST), Takasaki, Gunma, 370-1292, Japan.
Appl Radiat Isot. 2019 Sep;151:7-12. doi: 10.1016/j.apradiso.2019.05.034. Epub 2019 May 28.
Accurate analysis of N fixation in leguminous crops requires determination of N utilization within an intact plant; however, most approaches require tissue disassembly. We developed a simple and rapid technique to generate high-purity and high-yield [N]N gas and obtained real-time images of N fixation in an intact soybean plant. The purification efficiency was ∼81.6% after decay correction. Our method provides accurate signals of N fixation and allows free changes to the tracer gas composition to suit different experimental designs.
准确分析豆科作物中的固氮作用需要测定完整植株内的氮利用情况；然而，大多数方法都需要对组织进行拆解。我们开发了一种简单快速的技术来生成高纯度、高产率的[氮]氮气体，并获得了完整大豆植株中固氮作用的实时图像。经过衰变校正后，纯化效率约为81.6%。我们的方法提供了准确的固氮信号，并允许自由改变示踪气体成分以适应不同的实验设计。
