[肾包膜下测定作为一种化学敏感性试验(III)--宿主反应、实验性化疗及裸鼠应用的比较]

[Subrenal capsule assay as a chemosensitivity test (III)--Comparison of host reaction, experimental chemotherapy and use of nude mice].

作者信息

Kusuyama T, Fujita M, Origasa H, Shimozuma K, Fujita F, Taguchi T

出版信息

Gan To Kagaku Ryoho. 1987 Sep;14(9):2722-9.

Abstract

We studied fundamentally subrenal capsule assay, using human tumor specimens (gastric, breast and pancreas cancers) serially transplanted in nude mice. Any prominent difference of host reaction was not found between the host of BALB/c-nu/+, BALB/c-+/+ and CDF1 mice. Using immunocompetent BALB/c-nu/+ mice, experimental chemotherapy with mitomycin C (MMC) and 5-fluorouracil (5-FU) was carried out. On day 6, macroscopic and histological findings corresponded relatively well with 5-FU effect but not with MMC. Using BALB/c-nu/nu mice, we tried 15-day SRC assay. When the sensitivity of anti-cancer drugs was compared between early and intermediate phase after inoculation, no obvious difference was found macroscopically and histologically. BALB/c-nu/nu mouse will be useful as a host of SRC assay, and could be applicable to clinical fresh cases.

摘要

我们使用在裸鼠中连续移植的人类肿瘤标本（胃癌、乳腺癌和胰腺癌），对肾包膜下移植试验进行了基础研究。在BALB/c-nu/+、BALB/c-+/+和CDF1小鼠的宿主之间未发现宿主反应有任何显著差异。使用具有免疫活性的BALB/c-nu/+小鼠，进行了丝裂霉素C（MMC）和5-氟尿嘧啶（5-FU）的实验性化疗。在第6天，宏观和组织学结果与5-FU的效果相对吻合，但与MMC不吻合。使用BALB/c-nu/nu小鼠，我们尝试了为期15天的肾包膜下移植试验。当比较接种后早期和中期抗癌药物的敏感性时，在宏观和组织学上均未发现明显差异。BALB/c-nu/nu小鼠将作为肾包膜下移植试验的宿主有用，并且可应用于临床新鲜病例。