Department of Agricultural Biotechnology, Seoul National University, Seoul, 151-921, South Korea; Fertility Medical Center, Seoul Women's Hospital, Bucheon, 14544, South Korea.
Wide River Institute of Immunology, Seoul National University College of Medicine, Hongcheon, 25159, South Korea.
Biochem Biophys Res Commun. 2019 Jul 23;515(2):318-324. doi: 10.1016/j.bbrc.2019.05.153. Epub 2019 May 29.
Calcium (Ca) is an important element for many physiological functions of the uterus, including embryo implantation. Here, we investigated the possible involvement of altered intracellular Ca levels in decidualization in human endometrial stromal cells (hEMSCs). hEMSCs showed high levels of mesenchymal stem cell marker expression (CD73, CD90, and CD105) and did not express markers of hematopoietic progenitor cells (CD31, CD34, CD45, and HLA-DR). Decidualization is a process of ovarian steroid-induced endometrial stromal cell proliferation and differentiation. Several types of ion channels, which are regulated by the ovarian hormones progesterone and estradiol, as well as growth factors, are important for endometrial receptivity and embryo implantation. The combined application of progesterone (1 μM medroxyprogesterone acetate) and cyclic AMP (0.5 mM) for 6 days not only elevated inositol 1,4,5-triphosphate receptor (IPR)-mediated Ca release and IPR expression, it also promoted ORAI and STIM expression as well as cyclopiazonic acid-induced Ca release. Finally, intracellular Ca levels and ion channel gene expression influenced hEMSC proliferation. These results suggest that cytosolic Ca dynamics, mediated by specific ion channels, serve as an important step in the decidualization of hEMSCs.
钙（Ca）是子宫许多生理功能的重要元素，包括胚胎着床。在这里，我们研究了细胞内 Ca 水平改变在人子宫内膜基质细胞（hEMSCs）蜕膜化中的可能作用。hEMSCs 表现出高水平的间充质干细胞标志物表达（CD73、CD90 和 CD105），并且不表达造血祖细胞标志物（CD31、CD34、CD45 和 HLA-DR）。蜕膜化是卵巢甾体激素诱导的子宫内膜基质细胞增殖和分化的过程。几种类型的离子通道，受卵巢激素孕酮和雌二醇以及生长因子调节，对子宫内膜容受性和胚胎着床很重要。孕激素（1 μM 醋酸甲羟孕酮）和环磷酸腺苷（0.5 mM）联合应用 6 天，不仅提高了肌醇 1,4,5-三磷酸受体（IPR）介导的 Ca 释放和 IPR 表达，还促进了 ORAI 和 STIM 表达以及环匹阿尼酸诱导的 Ca 释放。最后，细胞内 Ca 水平和离子通道基因表达影响 hEMSC 的增殖。这些结果表明，特定离子通道介导的细胞质 Ca 动力学是 hEMSCs 蜕膜化的重要步骤。
