Graduate School of Engineering, Yokohama National University, 240-8501, Yokohama, Japan.
(b)Graduate School of Science and Engineering, Ehime University, 790-8577, Matsuyama, Japan.
Anal Biochem. 2019 Sep 1;580:14-20. doi: 10.1016/j.ab.2019.05.016. Epub 2019 May 30.
d-Serine is considered a key endogenous substance involved in several enzymatic reactions in the human body. In this study, solid-state vibrational circular dichroism (VCD) measurements of enantiomeric serine were performed for spectroscopic distinction between d- and l-serine. The mirror-image VCD signals of the isomers in the KBr pellets were observed in the mid-infrared range of 1800-1250 cm. The calculated infrared (IR) and VCD spectra for the optimized serine structures were in good agreement with the corresponding observed spectra. In addition, the chemical shift values estimated from the shielding constants of the optimized structure of serine corresponded with the observed values in C and N solid-state nuclear magnetic resonance spectra, supporting the VCD assignments. Our results suggest the need for further study of VCD to develop a sensitive and high-resolution spectroscopic technique for the detection of d-amino acids.
d-丝氨酸被认为是人体内参与多种酶反应的关键内源性物质。在这项研究中，通过固态振动圆二色性（VCD）测量对两种对映体丝氨酸进行了实验，以在光谱上区分 d-和 l-丝氨酸。在 KBr 压片中观察到了异构体在中红外区域 1800-1250cm 处的镜像 VCD 信号。优化丝氨酸结构的计算红外（IR）和 VCD 光谱与相应的观察光谱吻合良好。此外，从丝氨酸优化结构的屏蔽常数估算的化学位移值与 C 和 N 固态核磁共振谱中的观察值一致，支持 VCD 分配。我们的结果表明，需要进一步研究 VCD，以开发一种用于检测 d-氨基酸的灵敏、高分辨率的光谱技术。
