2nd Department of Int. Med Propaedeutic "Attikon" University Hospital, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
3rd Department of Obstetrics and Gynecology, "Attikon" University Hospital, Athens, Greece.
Hormones (Athens). 2019 Jun;18(2):173-178. doi: 10.1007/s42000-019-00111-x. Epub 2019 Jun 1.
To investigate DNA methylation changes in peripheral blood from patients with gestational diabetes mellitus (GDM) and preeclampsia (PE) due to poorly treated GDM.
Eighteen pregnant women participated in the study: 6 with GDM, 6 with PE, and 6 healthy controls. The promoter methylation status of genes was profiled using the Human Inflammatory Response and Autoimmunity EpiTect Methyl II Signature PCR Array profiles. The results were validated with quantitative real-time polymerase chain reaction (qRT-PCR).
Fewer inflammation-related genes were significantly hypomethylated in PE cases compared to healthy subjects than in GDM cases. Some of the examined genes show different methylation patterns between GDM and PE.
The epigenetic changes observed in this study indicate that GDM and PE exhibit specific DNA methylation profiles, with possible clinical applications.
研究由于治疗不佳的妊娠期糖尿病（GDM）导致的妊娠糖尿病（GDM）和先兆子痫（PE）患者外周血中的 DNA 甲基化变化。
18 名孕妇参与了这项研究：6 名 GDM 患者、6 名 PE 患者和 6 名健康对照组。使用 Human Inflammatory Response and Autoimmunity EpiTect Methyl II Signature PCR Array 谱分析基因启动子的甲基化状态。结果用实时定量聚合酶链反应（qRT-PCR）进行验证。
与健康对照组相比，PE 病例中与炎症相关的基因明显低甲基化的数量少于 GDM 病例。一些检查的基因在 GDM 和 PE 之间表现出不同的甲基化模式。
本研究观察到的表观遗传变化表明，GDM 和 PE 表现出特定的 DNA 甲基化谱，具有潜在的临床应用。
