Department of Chemical Engineering, Chungbuk National University, Cheongju 28644, Republic of Korea.
J Microbiol Biotechnol. 2019 Jun 28;29(6):905-912. doi: 10.4014/1904.04014.
Bioethanol has attracted much attention in recent decades as a sustainable and environmentally friendly alternative energy source. In this study, we compared the production of bioethanol by and at different initial concentrations of cellobiose and glucose. The results showed that can utilize both glucose and cellobiose, whereas can only utilize glucose. The ethanol yields were 43-51% from different initial concentrations of carbon source. In addition, different concentrations of microcrystalline cellulose (Avicel) were directly converted to ethanol by a combination of and two yeasts. Cellulose was first hydrolyzed by a fully enzymatic saccharification process using cellulases, and the reducing sugars and glucose produced during the process were further used as carbon source for bioethanol production by or . Sequential culture of and two yeasts revealed that was more efficient for bioconversion of sugars to ethanol than . When 20 g/l Avicel was used as a carbon source, the maximum reducing sugar, glucose, and ethanol yields were 42%, 26%, and 20%, respectively. The maximum concentrations of reducing sugar, glucose, and ethanol were 10.9, 8.57, and 5.95 g/l, respectively, at 120 h by the combination of and from 50 g/l Avicel.
在过去几十年中，生物乙醇作为一种可持续且环保的替代能源而备受关注。在本研究中，我们比较了 和 在不同初始浓度的纤维二糖和葡萄糖下生产生物乙醇的情况。结果表明， 可以同时利用葡萄糖和纤维二糖，而 只能利用葡萄糖。从不同初始碳源浓度下的乙醇产率为 43-51%。此外，通过 和两种酵母的组合，直接将不同浓度的微晶纤维素（微晶纤维素）转化为乙醇。纤维素首先通过使用纤维素酶的完全酶解糖化过程进行水解，在此过程中产生的还原糖和葡萄糖进一步用作 通过 或 生产生物乙醇的碳源。 和两种酵母的顺序培养表明， 比 更有效地将糖转化为乙醇。当 20 g/l 微晶纤维素作为碳源时，最大还原糖、葡萄糖和乙醇产率分别为 42%、26%和 20%。在 120 小时时，通过 和 从 50 g/l 微晶纤维素中，最大还原糖、葡萄糖和乙醇浓度分别为 10.9、8.57 和 5.95 g/l。
