Gan Huiyun, Du Jingdong, Ouyang Hong, Cheng Jie, Mao Huadong
From Department of Otolaryngology, RenHe Hospital of Three Gorges University, Yichang, Hubei, China.
From Department of Otolaryngology, RenHe Hospital of Three Gorges University, Yichang, Hubei, China.
Auris Nasus Larynx. 2020 Feb;47(1):84-89. doi: 10.1016/j.anl.2019.05.005. Epub 2019 May 30.
Interleukin-27 (IL-27) is a recently identified cytokine which plays both pro-inflammatory and anti-inflammatory effect in different diseases. However, its function in the pathogenesis of allergic rhinitis (AR) was not clear so far.
Quantitative PCR (qPCR) and enzyme-linked immunosorbent assay (ELISA) were performed to detect the expression of interleukin 27 (IL-27) and Th2 cytokines (IL-4, IL-5, IL-13) from 22 patients diagnosed with AR and 20 normal controls. Purified peripheral blood mononuclear cells (PBMCs) were prepared for in vitro experiment.
Serum mRNA and protein levels of IL-27 were down-regulated in AR patients compared with normal controls. The expression of IL-27 showed negative correlation with Th2 cytokines. In vitro study showed that IL-27 significantly inhibited Th2 cytokines expression from PBMCs.
Our results suggested that decreased IL-27 expression in AR were correlated with Th2 response. IL-27 may be used as potential target in the future treatment of AR.
白细胞介素-27（IL-27）是一种最近发现的细胞因子，在不同疾病中发挥促炎和抗炎作用。然而，其在变应性鼻炎（AR）发病机制中的作用目前尚不清楚。
采用定量聚合酶链反应（qPCR）和酶联免疫吸附测定（ELISA）检测22例AR患者和20例正常对照者白细胞介素27（IL-27）和Th2细胞因子（IL-4、IL-5、IL-13）的表达。制备纯化的外周血单个核细胞（PBMCs）用于体外实验。
与正常对照相比，AR患者血清中IL-27的mRNA和蛋白水平下调。IL-27的表达与Th2细胞因子呈负相关。体外研究表明，IL-27显著抑制PBMCs中Th2细胞因子的表达。
我们的结果表明，AR中IL-27表达降低与Th2反应相关。IL-27可能成为未来AR治疗的潜在靶点。
