Marenzoni M L, Calò P, Foiani G, Tossici S, Passantino G, Decaro N, Mandara M T
Department of Veterinary Medicine, University of Perugia, via S. Costanzo n. 4, Perugia, Italy.
Polo Neurologico Veterinario, via Fondo Ausa 48, Dogana, Republic of San Marino and.
J Comp Pathol. 2019 May;169:20-24. doi: 10.1016/j.jcpa.2019.03.005. Epub 2019 May 10.
A 4-month-old puppy died after showing intracranial signs a few days after a suspected viral enteritis. Grossly, the right cerebral hemisphere had a large irregular cavity external to the internal capsule. Histopathological examination revealed a cystic lesion in the right hemisphere and non-suppurative inflammation of the diencephalon and periaqueductal nervous tissue. Porencephaly associated with periventricular non-suppurative encephalitis was diagnosed. A nested polymerase chain reaction (PCR) identified the presence of parvovirus DNA in the brain and real-time PCR typed this as canine parvovirus (CPV) type 2a. Immunohistochemistry revealed the presence of CPV antigen in the cytoplasm of scattered cells in the subependymal layers and choroid plexus epithelium. The porencephaly was not associated with inflammatory lesions or CPV antigen and was considered to have preceded the neurological signs. In contrast, the detection of CPV antigen in the subependymal layers and choroid plexus epithelium supported the association of this virus with the periventricular encephalitis.
一只4个月大的幼犬在疑似病毒性肠炎几天后出现颅内症状，随后死亡。大体检查显示，右侧大脑半球在内囊外侧有一个大的不规则空洞。组织病理学检查发现右侧半球有一个囊性病变，间脑和导水管周围神经组织有非化脓性炎症。诊断为与脑室周围非化脓性脑炎相关的脑穿通畸形。巢式聚合酶链反应（PCR）检测到大脑中存在细小病毒DNA，实时PCR将其鉴定为犬细小病毒（CPV）2a型。免疫组织化学显示，室管膜下层和脉络丛上皮的散在细胞胞质中存在CPV抗原。脑穿通畸形与炎症病变或CPV抗原无关，被认为在神经症状出现之前就已存在。相比之下，在室管膜下层和脉络丛上皮中检测到CPV抗原，支持了该病毒与脑室周围脑炎的关联。
