Inoue Masafumi, Saito Rie, Kakita Akiyoshi, Tainaka Kazuki
Department of System Pathology for Neurological Disorders, Center for Bioresources, Brain Research Institute, Niigata University, 1-757 Asahimachidori, Chuo-ku, Niigata 951-8585, Japan.
Department of Pathology, Brain Research Institute, Niigata University, 1-757 Asahimachidori, Chuo-ku, Niigata 951-8585, Japan.
Bioorg Med Chem Lett. 2019 Aug 1;29(15):1886-1890. doi: 10.1016/j.bmcl.2019.05.049. Epub 2019 May 28.
Three-dimensional (3D) imaging based on chemical tissue clearing in the post-mortem human brain is a promising approach for stereoscopic understanding of central nervous system diseases. Especially, delipidation of lipid-rich white matter (WM) is a rate-determining step in human brain clearing by hydrophilic reagents. In this study, we described the rapid delipidation of WM by a 1,2-hexanediol (HxD)-based aqueous solution. HxD delipidation enabled rapid clearing of a formalin-fixed human brain specimen including the WM. Although harsh HxD delipidation was applied to the brain tissue, conventional pathological staining patterns and various types of antigenicity were sufficiently preserved. Furthermore, HxD delipidation was compatible with 3D imaging of fluorescently-labeled tissue samples. HxD delipidation could be useful in future 3D neuropathological diagnosis.
基于化学组织透明化的三维（3D）成像技术在尸检人脑研究中，是立体理解中枢神经系统疾病的一种很有前景的方法。特别是，富含脂质的白质（WM）的脱脂是亲水性试剂进行人脑透明化处理的限速步骤。在本研究中，我们描述了基于1,2 -己二醇（HxD）的水溶液对WM进行快速脱脂的方法。HxD脱脂能够快速透明化包括WM在内的福尔马林固定的人脑标本。尽管对脑组织采用了严苛的HxD脱脂处理，但传统病理染色模式和各种抗原性都得到了充分保留。此外，HxD脱脂与荧光标记组织样本的3D成像兼容。HxD脱脂在未来的3D神经病理学诊断中可能会很有用。
