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霍乱毒素与蓝氏贾第鞭毛虫的结合。

Binding of cholera toxin to Giardia lamblia.

作者信息

McCardell B A, Madden J M, Stanfield J T, Tall B D, Stephens M J

机构信息

Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204.

出版信息

J Clin Microbiol. 1987 Sep;25(9):1786-8. doi: 10.1128/jcm.25.9.1786-1788.1987.

DOI:10.1128/jcm.25.9.1786-1788.1987
PMID:3116038
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC269332/
Abstract

Binding of cholera toxin to Giardia lamblia was demonstrated by two slightly different methods: an immunofluorescence technique using antibody to cholera toxin and anti-rabbit immunoglobulin G conjugated to fluorescein isothiocyanate, and a one-step fluorescence method in which G. lamblia was incubated with the B subunit of cholera toxin conjugated to fluorescein isothiocyanate.

摘要

通过两种略有不同的方法证实了霍乱毒素与蓝氏贾第鞭毛虫的结合

一种免疫荧光技术，使用针对霍乱毒素的抗体以及与异硫氰酸荧光素偶联的抗兔免疫球蛋白G；另一种是一步荧光法，即将蓝氏贾第鞭毛虫与与异硫氰酸荧光素偶联的霍乱毒素B亚基一起孵育。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d98/269332/686c435bd7eb/jcm00093-0229-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d98/269332/686c435bd7eb/jcm00093-0229-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d98/269332/686c435bd7eb/jcm00093-0229-a.jpg

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本文引用的文献

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An outbreak of foodborne giardiasis.
N Engl J Med. 1981 Jan 1;304(1):24-8. doi: 10.1056/NEJM198101013040106.
2
Mammalian bile promotes growth of Giardia lamblia in axenic culture.哺乳动物胆汁可促进贾第虫在无菌培养中的生长。
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Changes in intestinal fluid and mucosal immune responses to cholera toxin in Giardia muris infection and binding of cholera toxin to Giardia muris trophozoites.微小隐孢子虫感染时肠道液体和对霍乱毒素的黏膜免疫反应的变化以及霍乱毒素与微小隐孢子虫滋养体的结合
Infect Immun. 1985 Oct;50(1):243-9. doi: 10.1128/iai.50.1.243-249.1985.
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Immunodiagnostic detection of Giardia antigen in faeces by a rapid visual enzyme-linked immunosorbent assay.通过快速可视酶联免疫吸附测定法对粪便中贾第虫抗原进行免疫诊断检测。
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Giardiasis in day-care centers: evidence of person-to-person transmission.
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Immunological cross-reactivity between a heat-labile enterotoxin(s) of Escherichia coli and subunits of Vibrio cholerae enterotoxin.大肠杆菌不耐热肠毒素与霍乱弧菌肠毒素亚基之间的免疫交叉反应性。
Infect Immun. 1978 Sep;21(3):1036-9. doi: 10.1128/iai.21.3.1036-1039.1978.
9
The clinical and laboratory diagnosis of giardiasis.贾第虫病的临床与实验室诊断
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Neutralization of Salmonella toxin-induced elongation of Chinese hamster ovary cells by cholera antitoxin.霍乱抗毒素对沙门氏菌毒素诱导的中国仓鼠卵巢细胞伸长的中和作用。
Infect Immun. 1977 Mar;15(3):988-92. doi: 10.1128/iai.15.3.988-992.1977.