霍乱毒素与蓝氏贾第鞭毛虫的结合。
Binding of cholera toxin to Giardia lamblia.
作者信息
McCardell B A, Madden J M, Stanfield J T, Tall B D, Stephens M J
机构信息
Division of Microbiology, Food and Drug Administration, Washington, D.C. 20204.
出版信息
J Clin Microbiol. 1987 Sep;25(9):1786-8. doi: 10.1128/jcm.25.9.1786-1788.1987.
Abstract
Binding of cholera toxin to Giardia lamblia was demonstrated by two slightly different methods: an immunofluorescence technique using antibody to cholera toxin and anti-rabbit immunoglobulin G conjugated to fluorescein isothiocyanate, and a one-step fluorescence method in which G. lamblia was incubated with the B subunit of cholera toxin conjugated to fluorescein isothiocyanate.
摘要
通过两种略有不同的方法证实了霍乱毒素与蓝氏贾第鞭毛虫的结合
一种免疫荧光技术，使用针对霍乱毒素的抗体以及与异硫氰酸荧光素偶联的抗兔免疫球蛋白G；另一种是一步荧光法，即将蓝氏贾第鞭毛虫与与异硫氰酸荧光素偶联的霍乱毒素B亚基一起孵育。
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