Denning S M, Tuck D T, Vollger L W, Springer T A, Singer K H, Haynes B F
Department of Medicine, Duke University School of Medicine, Durham, NC 27710.
J Immunol. 1987 Oct 15;139(8):2573-8.
Recent study of human thymocyte-thymic epithelial (TE) cell interactions has demonstrated that thymocytes bind to TE cells, and a consequence of this binding is the provision of accessory cell signals by TE cells for phytohemagglutinin (PHA)-induced mature thymocyte activation. In this paper we report on studies of the molecules involved in TE cell-dependent mature thymocyte activation. TE-thymocyte interactions necessary for PHA-induced thymocyte activation were inhibited by monoclonal antibodies against the cluster of differentiation (CD)2 antigen on thymocytes and lymphocyte function-associated (LFA)-3 antigen on TE cells. Inhibition of TE accessory cell signals by antibodies against CD2 (alpha CD2) and LFA-3 (alpha LFA-3) antigens occurred early on during thymocyte activation and prevented thymocyte interleukin 2 receptor expression. Further, alpha CD2 and alpha LFA-3 inhibited PHA-induced thymocyte activation in whole thymic explant cultures suggesting a significant role of the CD2 and LFA-3 antigens in thymocyte activation when accessory cell signals for PHA-induced thymocyte triggering were delivered by cells within an intact thymic microenvironment.
最近对人类胸腺细胞 - 胸腺上皮（TE）细胞相互作用的研究表明，胸腺细胞与TE细胞结合，这种结合的一个结果是TE细胞为植物血凝素（PHA）诱导的成熟胸腺细胞激活提供辅助细胞信号。在本文中，我们报告了对参与TE细胞依赖性成熟胸腺细胞激活的分子的研究。针对胸腺细胞上分化簇（CD）2抗原和TE细胞上淋巴细胞功能相关（LFA）-3抗原的单克隆抗体抑制了PHA诱导的胸腺细胞激活所需的TE - 胸腺细胞相互作用。抗CD2（αCD2）和LFA - 3（αLFA - 3）抗原的抗体对TE辅助细胞信号的抑制在胸腺细胞激活早期就发生了，并阻止了胸腺细胞白细胞介素2受体的表达。此外，αCD2和αLFA-3在整个胸腺外植体培养物中抑制了PHA诱导的胸腺细胞激活，这表明当PHA诱导的胸腺细胞触发的辅助细胞信号由完整胸腺微环境中的细胞传递时，CD2和LFA-3抗原在胸腺细胞激活中起着重要作用。
