Rapid expression of c-fos specific messenger RNA after wounding of a BALB/c-3T3 fibroblast monolayer.

A grid of damaged areas was introduced into serum-starved BALB/c-3T3 fibroblast monolayers. The expression of c-fos specific messenger RNA was examined by the use of the cytoplasmic dot hybridisation technique 15, 30 and 45 min after wounding. The expression of c-fos messenger RNA was highest after 30 min, while microglobulin was constantly expressed. These results show that the unspecific event of wounding induces the specific expression of c-fos messenger RNA, an effect similar to that of growth factors during serum stimulation.