Lee K H, Matsuda F, Kinashi T, Kodaira M, Honjo T
Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.
J Mol Biol. 1987 Jun 20;195(4):761-8. doi: 10.1016/0022-2836(87)90482-7.
We isolated and sequenced six variable-region (V) gene segments of the human immunoglobulin heavy-chain (H) using the V71-2 segment as probe. These VH segments were more than 90% homologous to each other and less than 65% homologous to members of the three known VH families. The VH fragments hybridized to an identical set of restriction fragments on Southern blots of human placenta DNA. The new family was designated as the VH-IV family. The complexity of the VH-IV family was estimated to be at least nine genes, of which the sequenced seven were functional genes. The VH-IV family is homologous (76%) to the mouse Vh36-60 family.
我们以V71 - 2片段为探针，分离并测序了人免疫球蛋白重链（H）的六个可变区（V）基因片段。这些VH片段彼此之间的同源性超过90%，与三个已知VH家族成员的同源性低于65%。VH片段与人胎盘DNA的Southern印迹上一组相同的限制性片段杂交。这个新家族被命名为VH - IV家族。VH - IV家族的复杂性估计至少有九个基因，其中已测序的七个是功能基因。VH - IV家族与小鼠Vh36 - 60家族同源（76%）。
