Brodeur P H, Riblet R
Eur J Immunol. 1984 Oct;14(10):922-30. doi: 10.1002/eji.1830141012.
Seven families of Igh-V genes have been defined by Southern blot analysis of genomic DNA from eighteen inbred strains of mice. Each of twenty-four cloned Vh genes hybridized to one of seven nonoverlapping sets of Eco RI restriction fragments. These families contain from 2 to approximately 40 hybridizing fragments. From these data we estimate that the mouse Igh-V locus consists of one hundred Vh genes. Genes within a Vh family share greater than 80% sequence homology while the sequence homology between families is generally less than 70%. There is extensive restriction fragment length polymorphism among the strains analyzed allowing the assignment of complete (Igh-V + Igh-C) Igh haplotypes for eighteen inbred mouse strains.
通过对18个近交系小鼠的基因组DNA进行Southern印迹分析，已确定了7个Igh - V基因家族。24个克隆的Vh基因中的每一个都与7组不重叠的Eco RI限制性片段中的一组杂交。这些家族包含2至约40个杂交片段。根据这些数据，我们估计小鼠Igh - V基因座由100个Vh基因组成。一个Vh家族内的基因具有超过80%的序列同源性，而不同家族之间的序列同源性通常小于70%。在所分析的品系之间存在广泛的限制性片段长度多态性，从而能够为18个近交小鼠品系确定完整的（Igh - V + Igh - C）Igh单倍型。
