Rondeau J M, Samama J P, Samama B, Barth P, Moras D, Biellmann J F
Laboratoire de Cristallographie Biologique, Institute de Biologie Moleculaire et Cellulaire du CNRS, Strasbourg, France.
J Mol Biol. 1987 Jun 20;195(4):945-8. doi: 10.1016/0022-2836(87)90498-0.
Crystals of pig lens aldose reductase have been grown from polyethylene glycol solutions at pH 6.2 and analysed by X-ray diffraction. Two crystal forms were obtained. The first belongs to space group P1 with unit cell dimensions a = 81.3 A, b = 85.9 A, c = 56.6 A, alpha = 102.3 degrees, beta = 103.3 degrees, gamma = 79.0 degrees, with four molecules in the unit cell related by a 222 non-crystallographic symmetry. The second crystal form is hexagonal. The space group is P6(2)22 with a = b = 101 A, c = 257 A and two molecules in the asymmetric unit. Both forms are suitable for X-ray structure analysis to better than 3 A resolution.
猪晶状体醛糖还原酶晶体已在pH 6.2的聚乙二醇溶液中生长，并通过X射线衍射进行分析。获得了两种晶体形式。第一种属于空间群P1，晶胞参数为a = 81.3 Å，b = 85.9 Å，c = 56.6 Å，α = 102.3°，β = 103.3°，γ = 79.0°，晶胞中有四个分子，通过222非晶体学对称性相关联。第二种晶体形式是六方晶系。空间群为P6(2)22，a = b = 101 Å，c = 257 Å，不对称单元中有两个分子。两种形式都适用于分辨率优于3 Å的X射线结构分析。
