State Key Laboratory of Urban and Regional Ecology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Beijing 100085, China.
State Key Laboratory of Urban and Regional Ecology, Research Centre for Eco-Environmental Sciences, Chinese Academy of Sciences, 18 Shuangqing Road, Beijing 100085, China; University of the Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, China.
J Hazard Mater. 2019 Sep 5;377:186-194. doi: 10.1016/j.jhazmat.2019.05.089. Epub 2019 May 28.
A diverse array of ARGs has been detected in the guts of soil fauna residing in farmland soil. Biochar has been widely used in farmland for soil remediation and improvement of soil quality; however, the effects of biochar amendment on the gut-associated ARGs of soil fauna remain unclear. In the present study, collembolans were cultivated in soils amended with 6 types of biochars. High-throughput qPCR was used to establish ARG profiles of the collembolan guts as well as the surrounding soils. A total of 73 and 162 subtypes of ARGs were detected in the collembolan guts and soils, respectively. Biochar amendment significantly altered the ARG compositions of the collembolan guts and soils, in a biochar quality-dependent manner. However, only manure-derived biochar, which contained elevated concentrations of heavy metals, increased the relative abundance of gut-associated ARGs. Changes in the gut microbial community, MGEs and biochar properties explained 84% of the total ARG variations in the collembolan guts. The findings of this study suggested that biochar properties should receive more attention, as high doses of heavy metals in biochar could increase the abundance of ARGs in collembolan guts, thereby contributing to the spread of ARGs in the environment through collembolan movement.
在农田土壤中栖息的土壤动物肠道中已经检测到了多种多样的 ARGs。生物炭已被广泛用于农田土壤修复和改善土壤质量；然而，生物炭改良对土壤动物肠道相关 ARGs 的影响尚不清楚。在本研究中，使用 6 种生物炭对跳虫进行了培养。使用高通量 qPCR 建立了跳虫肠道和周围土壤的 ARG 图谱。在跳虫肠道和土壤中分别检测到了 73 和 162 种亚型的 ARG。生物炭改良以生物炭质量依赖的方式显著改变了跳虫肠道和土壤中 ARG 的组成。然而，只有含有较高重金属浓度的粪肥衍生生物炭增加了肠道相关 ARGs 的相对丰度。肠道微生物群落、移动遗传元件和生物炭特性的变化解释了跳虫肠道中总 ARG 变异的 84%。本研究结果表明，生物炭特性应该受到更多关注，因为生物炭中高浓度的重金属可能会增加跳虫肠道中 ARGs 的丰度，从而通过跳虫的运动促进 ARGs 在环境中的传播。
